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Information Journal Paper

Title

THE USE OF INDIRECT IMMUNOPEROXIDASE ASSAY IN DIAGNOSIS OF TYPE A (H9N2) AVIAN INFLUENZA VIRUS ANTIGEN ON FROZEN TISSUE SECTIONS

Pages

  11-21

Abstract

 AVIAN INFLUENZA (AI) is a viral disease of poultry worldwide and earlier DIAGNOSIS of the disease is very important in control programs. Among IMMUNOHISTOCHEMICAL STAINING TECHNIQUES, the poultry laboratory diagnosticians have used indirect immunoperoxidase (IIP) assay for rapid DIAGNOSIS of avian viral diseases. In this study the IIP assay was optimized, then compared with the standard AI virus (AIV) isolation procedure on trachea, lung and kidney tissue samples. The samples were prepared from broiler chicken experimentally infected with the reference H9N2 subtype of AIV as well as clinical specimens. For preparation of frozen tissue sections samples were embedded in OCT compound and put rapidly in liquid nitrogen and kept at -70°C. The tissue sections were fixed and subjected to IIP assay by using a type A specific polyclonal and goat anti-chicken peroxides conjugated antibodies. The sensitivity, specificity, positive and negative predication values IIP assay was 78, 90, 80 and 88% respectively.  

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    • Cite

    APA: Copy

    GHARAGOUZLOU, M., POURBAKHSH, S.A., BAHMANINEJAD, M., KHAZRAEINIA, P., VASFI MARANDI, M., & SHAMS ALDINI, M.. (2002). THE USE OF INDIRECT IMMUNOPEROXIDASE ASSAY IN DIAGNOSIS OF TYPE A (H9N2) AVIAN INFLUENZA VIRUS ANTIGEN ON FROZEN TISSUE SECTIONS. ARCHIVES OF RAZI INSTITUTE, -(53), 11-21. SID. https://sid.ir/paper/528478/en

    Vancouver: Copy

    GHARAGOUZLOU M., POURBAKHSH S.A., BAHMANINEJAD M., KHAZRAEINIA P., VASFI MARANDI M., SHAMS ALDINI M.. THE USE OF INDIRECT IMMUNOPEROXIDASE ASSAY IN DIAGNOSIS OF TYPE A (H9N2) AVIAN INFLUENZA VIRUS ANTIGEN ON FROZEN TISSUE SECTIONS. ARCHIVES OF RAZI INSTITUTE[Internet]. 2002;-(53):11-21. Available from: https://sid.ir/paper/528478/en

    IEEE: Copy

    M. GHARAGOUZLOU, S.A. POURBAKHSH, M. BAHMANINEJAD, P. KHAZRAEINIA, M. VASFI MARANDI, and M. SHAMS ALDINI, “THE USE OF INDIRECT IMMUNOPEROXIDASE ASSAY IN DIAGNOSIS OF TYPE A (H9N2) AVIAN INFLUENZA VIRUS ANTIGEN ON FROZEN TISSUE SECTIONS,” ARCHIVES OF RAZI INSTITUTE, vol. -, no. 53, pp. 11–21, 2002, [Online]. Available: https://sid.ir/paper/528478/en

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