THE ROLE OF BACTERIAL VECTORS IN THE EXPRESSION OF HUMAN IFN- Γ GENE

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MOEEN REZAKHANLOU A.; MAHBOUDI F.; MAGHSOUDI N.; HABIBI GHOLAM REZA

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Journal Paper

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Journal: ARCHIVES OF RAZI INSTITUTE Year:2003 | Volume:- | Issue:55 Page(s): 71-80

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Title

THE ROLE OF BACTERIAL VECTORS IN THE EXPRESSION OF HUMAN IFN- Γ GENE

Author(s)

MOEEN REZAKHANLOU A. | MAHBOUDI F. | MAGHSOUDI N. | HABIBI GHOLAM REZA | Issue Writer Certificate

Keywords

INTERFERON-Γ

EXPRESSION

E. COLI

NORTHERN BLOTTING

Abstract

The Interferon gamma (IFN-γ) gene 1 was isolated from phytohemagglutinin stimulated peripheral blood mononuclear cells of a healthy individual blood donor using RT-PCR technique. The gene was cloned under the control of the different promoters EXPRESSION vectors such as pET32a (Novagen), pQE30 (QIAGEN), pSKA-IBA (Strep-Tag), pRSET (Invitrogen), and then expressed in Ecoli. The transcription of IFN- γ mRNA was determined by northern blot analysis. The level of EXPRESSION of human IFN-γ in pRSET vector under the control of T7 promoter was determined by laser-based densitometry of SDSPAGE and found more than 26% of total bacterial protein. The EXPRESSION was confirmed by western blotting. The EXPRESSION of IFN-γ under the control of promoters of pET32a, pQE30 and pSKA-IBA plasmids was detected by SDS-GAGE and western blotting.

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APA: Copy

MOEEN REZAKHANLOU, A., MAHBOUDI, F., MAGHSOUDI, N., & HABIBI, GHOLAM REZA. (2003). THE ROLE OF BACTERIAL VECTORS IN THE EXPRESSION OF HUMAN IFN- Γ GENE. ARCHIVES OF RAZI INSTITUTE, -(55), 71-80. SID. https://sid.ir/paper/528571/en

Vancouver: Copy

MOEEN REZAKHANLOU A., MAHBOUDI F., MAGHSOUDI N., HABIBI GHOLAM REZA. THE ROLE OF BACTERIAL VECTORS IN THE EXPRESSION OF HUMAN IFN- Γ GENE. ARCHIVES OF RAZI INSTITUTE[Internet]. 2003;-(55):71-80. Available from: https://sid.ir/paper/528571/en

IEEE: Copy

A. MOEEN REZAKHANLOU, F. MAHBOUDI, N. MAGHSOUDI, and GHOLAM REZA HABIBI, “THE ROLE OF BACTERIAL VECTORS IN THE EXPRESSION OF HUMAN IFN- Γ GENE,” ARCHIVES OF RAZI INSTITUTE, vol. -, no. 55, pp. 71–80, 2003, [Online]. Available: https://sid.ir/paper/528571/en

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