STANDARDIZATION OF ELISA FOR DETECTION OF AVIAN INFLUENZA VIRUS ANTIBODIES

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POURBAKHSH S.A.; KIANIZADEH M.; MOUSAKHANI F.

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Journal: ARCHIVES OF RAZI INSTITUTE Year:1999 | Volume:- | Issue:50 Page(s): 0-0

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Title

STANDARDIZATION OF ELISA FOR DETECTION OF AVIAN INFLUENZA VIRUS ANTIBODIES

Author(s)

POURBAKHSH S.A. | KIANIZADEH M. | MOUSAKHANI F. | Issue Writer Certificate

Keywords

AVIAN INFLUENZA

ELISA

ANTIBODY

Abstract

An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the rapid and efficient large scale screening of antibodies to AVIAN INFLUENZA virus (AIV) infection in chicken. Antigen was a whole-purified influenza virus produced from the H9N2 subtype. Optimum dilution for goat anti-chicken conjugate to be used in the ELISA was 1:1000, as determined by signal-to-noise ratio. The antigen concentration was 0.375μg of protein per well, as determined by checkerboard titration. The sensitivity of the ELISA was compared with hemmaglutination inhibition test under field exposure. After testing of 656 field sera, the correlation coefficient for the results of two tests was significant (r=0.929, P<0.001). Testing 8 standard antisera of various subtypes (H1, H2, H4, H5, H6, H7, H9 and H10) of AIV and AIV ANTIBODY positive and negative sera determined specificity of the ELISA. Antisera to all 8-hemmaglutinin subtypes were strongly positive. Both the sensitivity and specificity of the ELISA was compared to the other test. Thus, the ELISA was able to detect specific AIV antibodies and suitable for screening large numbers of samples in diagnostic laboratories.

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APA: Copy

POURBAKHSH, S.A., KIANIZADEH, M., & MOUSAKHANI, F.. (1999). STANDARDIZATION OF ELISA FOR DETECTION OF AVIAN INFLUENZA VIRUS ANTIBODIES. ARCHIVES OF RAZI INSTITUTE, -(50), 0-0. SID. https://sid.ir/paper/530226/en

Vancouver: Copy

POURBAKHSH S.A., KIANIZADEH M., MOUSAKHANI F.. STANDARDIZATION OF ELISA FOR DETECTION OF AVIAN INFLUENZA VIRUS ANTIBODIES. ARCHIVES OF RAZI INSTITUTE[Internet]. 1999;-(50):0-0. Available from: https://sid.ir/paper/530226/en

IEEE: Copy

S.A. POURBAKHSH, M. KIANIZADEH, and F. MOUSAKHANI, “STANDARDIZATION OF ELISA FOR DETECTION OF AVIAN INFLUENZA VIRUS ANTIBODIES,” ARCHIVES OF RAZI INSTITUTE, vol. -, no. 50, pp. 0–0, 1999, [Online]. Available: https://sid.ir/paper/530226/en

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