POLYACRYLAMIDE ABILITY FOR PROTEIN IMMOBILIZATION IN ONE-PHASE BINARY-SOLVENT SYSTEMS

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HAGH BEIN K.; AGHA RAFEIE R.; MAGHSOUDI S.

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Journal Paper

Paper Information

Journal: Iranian Polymer Journal Year:2005 | Volume:14 | Issue:8 (62) Page(s): 729-734

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Title

POLYACRYLAMIDE ABILITY FOR PROTEIN IMMOBILIZATION IN ONE-PHASE BINARY-SOLVENT SYSTEMS

Author(s)

HAGH BEIN K. | AGHA RAFEIE R. | MAGHSOUDI S. | Issue Writer Certificate

Keywords

PROTEIN IMMOBILIZATION

CROSS-LINKED POLYACRYLAMIDE

AMPHIPHILIC STRUCTURE

BINARY-SOLVENT SYSTEMS

Abstract

The PROTEIN IMMOBILIZATION ability of CROSS-LINKED POLYACRYLAMIDE (CLP) was studied in one-phase BINARY-SOLVENT SYSTEMS. Four miscible solvents in water; 2 protic (methanol, 2-propanol) and 2 aprotic (THF, acetonitrile), and bovine serum albumin (BSA) as target protein were chosen. According to the results of a study on the sieve structure of CLP, the polymer which was made from 1.69 M acrylamide and 2.03 ×10-2 M bis-acryl amide was selected for the research. Results showed that CLP immobilization efficiency increased as the co-solvent share in the binary-solvents increased. However, it was not necessary to omit water. The immobilization efficiency of CLP was satisfying in the binary systems containing about 50% 2-propanol or acetonitrile, or 70% Methanol or THF. The CLP immobilization performance was very high when the organic solvent share was increased above 90% in all the studied binary media. The outcome suggests that the tolerable level of water and the role of co-solvent in the applied system are mainly affected by the amphiphilic nature of CLP. Assumingly, the AMPHIPHILIC STRUCTURE helps CLP to be applied in one-phase binary systems containing co-solvent from various groups of protic or aprotic with different log P.

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APA: Copy

HAGH BEIN, K., AGHA RAFEIE, R., & MAGHSOUDI, S.. (2005). POLYACRYLAMIDE ABILITY FOR PROTEIN IMMOBILIZATION IN ONE-PHASE BINARY-SOLVENT SYSTEMS . IRANIAN POLYMER JOURNAL (ENGLISH), 14(8 (62)), 729-734. SID. https://sid.ir/paper/530751/en

Vancouver: Copy

HAGH BEIN K., AGHA RAFEIE R., MAGHSOUDI S.. POLYACRYLAMIDE ABILITY FOR PROTEIN IMMOBILIZATION IN ONE-PHASE BINARY-SOLVENT SYSTEMS . IRANIAN POLYMER JOURNAL (ENGLISH)[Internet]. 2005;14(8 (62)):729-734. Available from: https://sid.ir/paper/530751/en

IEEE: Copy

K. HAGH BEIN, R. AGHA RAFEIE, and S. MAGHSOUDI, “POLYACRYLAMIDE ABILITY FOR PROTEIN IMMOBILIZATION IN ONE-PHASE BINARY-SOLVENT SYSTEMS ,” IRANIAN POLYMER JOURNAL (ENGLISH), vol. 14, no. 8 (62), pp. 729–734, 2005, [Online]. Available: https://sid.ir/paper/530751/en

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