REAL-TIME PCR METHOD AND APPLICATIONS IN PLANT PATHOLOGY

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SHARIFI R.; RASHIDI MONFARED S.

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Journal Paper

Paper Information

Journal: MODERN GENETICS JOURNAL (MGJ) Year:2007 | Volume:- | Issue:6-7 Page(s): 40-45

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Information Journal Paper

Title

REAL-TIME PCR METHOD AND APPLICATIONS IN PLANT PATHOLOGY

Author(s)

SHARIFI R. | RASHIDI MONFARED S. | Issue Writer Certificate

Keywords

GENE EXPRESSION

REAL- TIME PCR

PLANT PATHOGENS

Abstract

In 1992, the analysis of PCR kinetics becomes possible by constructing a system that detects PCR products as they accumulate. This "realtime" system includes the intercalator ethidium bromide in each amplification reaction, an adapted thermal cycler to irradiate the samples with ultraviolet light and detection of the resulting fluorescence with a computer-controlled cooled CCD camera. Amplification produces increasing amounts of double-stranded DNA, which binds ethidium bromide, resulting in an increase in fluorescence. By plotting the increase in fluorescence versus cycle number, the system produces amplification plots that provide a more complete picture of the PCR process than assaying product accumulation after a fixed number of cycles. This methodology could be used to quantify population of beneficial soil bacteria and fungi in root or rhizospher of host plant for example ammonia-oxidizing bacteria, siderophore production bacteria and mycorrhiza as well as the investigation of therapeutic effect of different pesticide on the plant tissue, study of expression of different gene under environment stress condition the study of mechanism of resistance plants against PLANT PATHOGENS, mutation is induced in organism genomes.

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APA: Copy

SHARIFI, R., & RASHIDI MONFARED, S.. (2007). REAL-TIME PCR METHOD AND APPLICATIONS IN PLANT PATHOLOGY. MODERN GENETICS JOURNAL (MGJ), -(6-7), 40-45. SID. https://sid.ir/paper/532488/en

Vancouver: Copy

SHARIFI R., RASHIDI MONFARED S.. REAL-TIME PCR METHOD AND APPLICATIONS IN PLANT PATHOLOGY. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2007;-(6-7):40-45. Available from: https://sid.ir/paper/532488/en

IEEE: Copy

R. SHARIFI, and S. RASHIDI MONFARED, “REAL-TIME PCR METHOD AND APPLICATIONS IN PLANT PATHOLOGY,” MODERN GENETICS JOURNAL (MGJ), vol. -, no. 6-7, pp. 40–45, 2007, [Online]. Available: https://sid.ir/paper/532488/en

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