STANDARDIZATION OF AN ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF INFECTIOUS BRONCHITIS VIRUS ANTIBODY

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

GHADAKCHI H.; DADRAS H.; POURBAKHSH S.A.; HOSSEINI S.M.H.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: ARCHIVES OF RAZI INSTITUTE Year:2005 | Volume:- | Issue:59 Page(s): 75-83

Download Full-Text

View:

371

Download:

365

Cites:

Information Journal Paper

Title

STANDARDIZATION OF AN ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF INFECTIOUS BRONCHITIS VIRUS ANTIBODY

Author(s)

GHADAKCHI H. | DADRAS H. | POURBAKHSH S.A. | HOSSEINI S.M.H. | Issue Writer Certificate

Keywords

INFECTIOUS BRONCHITIS

ELISA

ANTIBODY

MASSACHUSETTS SEROTYPE

Abstract

An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of ANTIBODY to avian INFECTIOUS BRONCHITIS virus (IBV). Antigen was prepared from whole-purified IBV MASSACHUSETTS SEROTYPE (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respectively. The optical densities (OD) were compared with a commercial ELISA kit. Also, the results of ELISA were compared to hemagglutination inhibition (HI) test. The correlation coefficient of the results of both ELISAs and HI was significant (P<0.05). The developed ELISA can detect ANTIBODY against IBV and is more sensitive and suitable for screening of samples in diagnostic laboratories.

Cites

No record.

References

No record.

Cite

APA: Copy

GHADAKCHI, H., DADRAS, H., POURBAKHSH, S.A., & HOSSEINI, S.M.H.. (2005). STANDARDIZATION OF AN ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF INFECTIOUS BRONCHITIS VIRUS ANTIBODY. ARCHIVES OF RAZI INSTITUTE, -(59), 75-83. SID. https://sid.ir/paper/534444/en

Vancouver: Copy

GHADAKCHI H., DADRAS H., POURBAKHSH S.A., HOSSEINI S.M.H.. STANDARDIZATION OF AN ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF INFECTIOUS BRONCHITIS VIRUS ANTIBODY. ARCHIVES OF RAZI INSTITUTE[Internet]. 2005;-(59):75-83. Available from: https://sid.ir/paper/534444/en

IEEE: Copy

H. GHADAKCHI, H. DADRAS, S.A. POURBAKHSH, and S.M.H. HOSSEINI, “STANDARDIZATION OF AN ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF INFECTIOUS BRONCHITIS VIRUS ANTIBODY,” ARCHIVES OF RAZI INSTITUTE, vol. -, no. 59, pp. 75–83, 2005, [Online]. Available: https://sid.ir/paper/534444/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops

No record.