DETECTION AND CHARACTERIZATION OF A DOUBLE-STRANDED RNA MYCOVIRUS IN FUSARIUM OXYSPORUM F. SP. MELONIS

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SHARZEHEI A.; BANIHASHEMI Z.A.D.; AFSHARIFAR A.R.

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Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF PLANT PATHOLOGY Year:2007 | Volume:43 | Issue:1 (169) Page(s): 9-26

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Title

DETECTION AND CHARACTERIZATION OF A DOUBLE-STRANDED RNA MYCOVIRUS IN FUSARIUM OXYSPORUM F. SP. MELONIS

Author(s)

SHARZEHEI A. | BANIHASHEMI Z.A.D. | AFSHARIFAR A.R. | Issue Writer Certificate

Keywords

FUSARIUM OXYSPORUM F. SP. MELONIS

DSRNA

MYCOVIRUS

CHRYSOVIRUS

CUCUMIS MELO

Abstract

FUSARIUM OXYSPORUM F. SP. MELONIS, the causal agent of Fusarium wilt of CUCUMIS MELO was investigated for the presence of MYCOVIRUSes. Four DSRNA segments were identified in an isolate of F. oxysporum isolated from stems of CUCUMIS MELO plants in Maharloo (east of Shiraz). DNase and RNase treatments confirmed the double-stranded nature of the RNA molecules. Mycelial mats were obtained in PD broth. The DSRNAs were extracted and purified using CF-11 cellulose chromatography. The DSRNA segments were approximately 0.7, 2.3, 2.5 and 3 kbp in size. Virus purification and electron microscopy revealed the presence of isometric virus-like particles (VLPs), ca 35 nm in diameter. The name FUSARIUM OXYSPORUM F. SP. MELONIS virus (FuOMV) was suggested for this virus. Attempts to cure of DSRNA from the viruliferous culture by hyphal tip excision or using a range of cycloheximide concentrations, up to 200 mg ml-1, with or without incubation at 32°C were unsuccessful. Pathogenecity tests by root dip inoculation of susceptible C. melo cultivars with a suspension of 5x105 conidia per ml indicated the virulence of this isolate. The results implied that the isolated DSRNAs have no apparent effects on morphology and pathogenicity of the F. oxysporum f. sp. melonis. Molecular cloning and partial nucleotide sequencing of the largest DSRNA segment (dsRNA I) showed the significant sequence similarity of the deduced amino acid sequences to the RdRp encoded by CHRYSOVIRUSes. Collectively, the biological and molecular data imply the resemblance of this MYCOVIRUS with those of CHRYSOVIRUS genus.

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APA: Copy

SHARZEHEI, A., BANIHASHEMI, Z.A.D., & AFSHARIFAR, A.R.. (2007). DETECTION AND CHARACTERIZATION OF A DOUBLE-STRANDED RNA MYCOVIRUS IN FUSARIUM OXYSPORUM F. SP. MELONIS. IRANIAN JOURNAL OF PLANT PATHOLOGY, 43(1 (169)), 9-26. SID. https://sid.ir/paper/553635/en

Vancouver: Copy

SHARZEHEI A., BANIHASHEMI Z.A.D., AFSHARIFAR A.R.. DETECTION AND CHARACTERIZATION OF A DOUBLE-STRANDED RNA MYCOVIRUS IN FUSARIUM OXYSPORUM F. SP. MELONIS. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2007;43(1 (169)):9-26. Available from: https://sid.ir/paper/553635/en

IEEE: Copy

A. SHARZEHEI, Z.A.D. BANIHASHEMI, and A.R. AFSHARIFAR, “DETECTION AND CHARACTERIZATION OF A DOUBLE-STRANDED RNA MYCOVIRUS IN FUSARIUM OXYSPORUM F. SP. MELONIS,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 43, no. 1 (169), pp. 9–26, 2007, [Online]. Available: https://sid.ir/paper/553635/en

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