DETECTION OF CTX-M-1 BETA LACTAMASE GENE IN ESCHERICHIA COLI ISOLATED FROM CLINICAL SAMPLES BY POLYMERASE CHAIN REACTION (PCR)

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SOLTAN DALLAL MOHAMMAD MEHDI; MOBASSERI GOLNAZ; FALLAH MEHRABADI JALIL; ESHRAGHIAN MOHAMMADREZA; RASTEGAR LARI ABDOLAZIZ; AGHAMIRZAEI HEDROSHA MOLLA; SABBAGHI AYLAR; AZARSA MOHAMMAD

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Journal: AMIRKABIR Year:2011 | Volume:69 | Issue:1 Page(s): 16-20

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Title

DETECTION OF CTX-M-1 BETA LACTAMASE GENE IN ESCHERICHIA COLI ISOLATED FROM CLINICAL SAMPLES BY POLYMERASE CHAIN REACTION (PCR)

Author(s)

Keywords

E. COLI

EXTENDED SPECTRUM BETA LACTAMASE

CTX-M-1

Abstract

Background: Resistance to beta-lactam antibiotics in clinical isolates frequently results from the production of £]-lactamase enzymes. In recent years, the production of extended spectrum £]-lactamases (ESBLs) and AmpC £]-lactamase have greatly increased, especially in clinically isolated Escherichia coli. On the other hand, betalactamase genes have several subfamilies and designing universal primers could be valuable in their detection. The beta-lactamase-producing E. COLI which is resistant to beta-lactam antibiotics may pose a great risk to the patients. The CTX-M-1 gene is responsible for beta lactamase resistance. The purpose of this study was to find the percentage of CTX-M-1 carrying E. COLI strains.Methods: A total of 500 urine samples were collected from different hospitals in Tehran, Iran during September to February 2009. The samples were cultured on EMB agar and incubated at 37„a C for 24 hours. Some biochemical tests were carried out on the isolated samples. The presence of CTX-M-1 gene was determined by PCR on the isolates already identified phenotypically by disk diffusion agar and combined disks.Results: In general, 200 out of the initial 500 samples were identified as E. COLI, among which 128 (79.5%) were ESBLs producing strains. PCR used for the detection of CTX-M- 1 gene, showed that 99 (77.34%) out of 128 isolates contained such gene.Conclusion: The results of this study showed a high percentage of £]-lactamase resistant E. COLI strains. This is a serious matter and would pose a public hazard and every step should be taken to avoid such hazard.

Cites

PREVALENCE OF BETA-LACTAMASES GENES AND ANTIBIOTIC RESISTANCE PATTERN OF KLEBSIELLA PNEUMONIAE ISOLATED FROM TEACHING HOSPITALS, SARI, IRAN, 2014

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APA: Copy

SOLTAN DALLAL, MOHAMMAD MEHDI, MOBASSERI, GOLNAZ, FALLAH MEHRABADI, JALIL, ESHRAGHIAN, MOHAMMADREZA, RASTEGAR LARI, ABDOLAZIZ, AGHAMIRZAEI, HEDROSHA MOLLA, SABBAGHI, AYLAR, & AZARSA, MOHAMMAD. (2011). DETECTION OF CTX-M-1 BETA LACTAMASE GENE IN ESCHERICHIA COLI ISOLATED FROM CLINICAL SAMPLES BY POLYMERASE CHAIN REACTION (PCR). AMIRKABIR, 69(1), 16-20. SID. https://sid.ir/paper/563517/en

Vancouver: Copy

SOLTAN DALLAL MOHAMMAD MEHDI, MOBASSERI GOLNAZ, FALLAH MEHRABADI JALIL, ESHRAGHIAN MOHAMMADREZA, RASTEGAR LARI ABDOLAZIZ, AGHAMIRZAEI HEDROSHA MOLLA, SABBAGHI AYLAR, AZARSA MOHAMMAD. DETECTION OF CTX-M-1 BETA LACTAMASE GENE IN ESCHERICHIA COLI ISOLATED FROM CLINICAL SAMPLES BY POLYMERASE CHAIN REACTION (PCR). AMIRKABIR[Internet]. 2011;69(1):16-20. Available from: https://sid.ir/paper/563517/en

IEEE: Copy

MOHAMMAD MEHDI SOLTAN DALLAL, GOLNAZ MOBASSERI, JALIL FALLAH MEHRABADI, MOHAMMADREZA ESHRAGHIAN, ABDOLAZIZ RASTEGAR LARI, HEDROSHA MOLLA AGHAMIRZAEI, AYLAR SABBAGHI, and MOHAMMAD AZARSA, “DETECTION OF CTX-M-1 BETA LACTAMASE GENE IN ESCHERICHIA COLI ISOLATED FROM CLINICAL SAMPLES BY POLYMERASE CHAIN REACTION (PCR),” AMIRKABIR, vol. 69, no. 1, pp. 16–20, 2011, [Online]. Available: https://sid.ir/paper/563517/en

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