OPTIMIZATION OF CALLUS INDUCTION AND REGENERATION MEDIA FOR IRANIAN CULTIVATED RICE(ORYZA SATIVA L.)

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MOHAMMADI A.; TABATABAEI B.A.S.

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Journal: Year:2008 | Volume:13 | Issue:2 Page(s): 365-374

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Title

OPTIMIZATION OF CALLUS INDUCTION AND REGENERATION MEDIA FOR IRANIAN CULTIVATED RICE(ORYZA SATIVA L.)

Author(s)

MOHAMMADI A. | TABATABAEI B.A.S. | Issue Writer Certificate

Keywords

CALLUS INDUCTION

REGENERATION

RICE

CARBON SOURCE

Abstract

For introduction of foreign genes to main crops such as RICE, we need an efficient and reproducible REGENERATION system yielding fertile plants from transformed tissues.For this intention, we established the best CALLUS INDUCTION medium for Tarom mahalli and Deilamani cultivars and created the method that the REGENERATION frequency was reached to 49%. Callus was initiated from mature embryos of this cultivars on MS medium supplemented with 3 level of 2, 4-D (2, 2.5 and 3 mg.1-1) and N6 medium supplemented with 5 level of 2, 4-D (1.5, 2, 2.5, 3 and 3.5 mg..1-1)). We analyzed this data with CRD design, the number, mass and morphology of the callus formed on the scutellum were dependent on the medium used. As a result, for both cultivar the best medium were MS supplemented with 2 mg.-112, 4-D. In subculture media, sucrose was used instead of maltose. For determining of best media for REGENERATION of plantlets, we stabilished factorial experiment with 3 factor: REGENERATION media (6 level): MS medium supplemented with 5 amount of kinetin and: NAA (mg.r) [(6& 2), (4& 2), (2& 0.5), (3& 1), (2& 1) respectively] and 0.2 mg.-1 2, 4-D and 2 mg.1-1BAP, CARBON SOURCE with 2 level (sucrose and mannitol) and genotype with 2 level (Tarom mahali and Deilamani). RICE calli were dehydrated with two layers of filter paper for 30 minutes (prior to transfer to the REGENERATION media). As a result, the best REGENERATION medium for both cultivar is MS suplemented with 3mg.1-1 Kin, 1 mg.1-1 NAA and mannitol as a CARBON SOURCE.

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APA: Copy

MOHAMMADI, A., & TABATABAEI, B.A.S.. (2008). OPTIMIZATION OF CALLUS INDUCTION AND REGENERATION MEDIA FOR IRANIAN CULTIVATED RICE(ORYZA SATIVA L.). JOURNAL OF AGRICULTURAL SCIENCES, 13(2), 365-374. SID. https://sid.ir/paper/565484/en

Vancouver: Copy

MOHAMMADI A., TABATABAEI B.A.S.. OPTIMIZATION OF CALLUS INDUCTION AND REGENERATION MEDIA FOR IRANIAN CULTIVATED RICE(ORYZA SATIVA L.). JOURNAL OF AGRICULTURAL SCIENCES[Internet]. 2008;13(2):365-374. Available from: https://sid.ir/paper/565484/en

IEEE: Copy

A. MOHAMMADI, and B.A.S. TABATABAEI, “OPTIMIZATION OF CALLUS INDUCTION AND REGENERATION MEDIA FOR IRANIAN CULTIVATED RICE(ORYZA SATIVA L.),” JOURNAL OF AGRICULTURAL SCIENCES, vol. 13, no. 2, pp. 365–374, 2008, [Online]. Available: https://sid.ir/paper/565484/en

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