DETECTION OF BORNA DISEASE VIRAL ANTIGEN (BDV-P24) IN PBMCS AS WELL AS ANTIBODIES AGAINST BDV-P24 AND BDV-P40 PROTEINS OF SERUM SAMPLES

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RAD M.A.; NAKAYA T.

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Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR) Year:2001 | Volume:2 | Issue:2 Page(s): 207-193

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Title

DETECTION OF BORNA DISEASE VIRAL ANTIGEN (BDV-P24) IN PBMCS AS WELL AS ANTIBODIES AGAINST BDV-P24 AND BDV-P40 PROTEINS OF SERUM SAMPLES

Author(s)

RAD M.A. | NAKAYA T. | Issue Writer Certificate

Keywords

BOMA DISEASE

BDV-P24

BDV-P40

RNA

IMMUNOBLOT

RAD

IRAN

Abstract

The aim of this study was to find out the epidemiological status of Borna disease in two limited groups of people in IRAN following the previous study of varied prevalence of Borna disease virus (BDV) infection in horses of IRAN (Bahmani et al., 1996).Thirty blood samples were collected from healthy people selected among the people who had contact with apparently healthy horses, but carriers of BDV for many years. These people were called group "A" Thirty blood samples were also collected from the volunteer blood donors of National Blood Transfusion Organization of IRAN. These healthy people were called group "B". All volunteers in group A were male except one female. All volunteers in group "B" were male.Total cellular RNA was extracted from peripheral blood mononuclear cells (PBMC) of the above mentioned blood samples. The extracted RNA was amplified and transcribed to cDNA by a nested reverse transcriptase polymerase chain reaction (RT-PCR) to obtain a fragment of the p24 coding region which is relatively conserved within the BDV-RNA genome. Using specific Marker in agarose gel electrophoresis technique, the positive protein bands could be visualized at 391 base pair molecular weight when were caused to shine by ultra violet light in the gel-agar, conjugated with ethydium bromide.The molecular weight of BDV-P24 KDa at 391 base pair could be identified when it was compared with the Marker band (fi-X-174 Hae-III) which was applied in the test. An MDCK (Medin Darby canine kidney) cell persistently infected with BDV was used as positive control and distilled water was used as negative control in each of the serial tests in gel electrophoresis technique.The results were confirmed by Southern blot hybridizationtest using nucleic acid transfer membrane and conjugated oligo nucleotide cDNA with [32 P]- ATP RADio isotope. The PBMCs in the people of group "A" showed 26.66% positive reactions for BDV-P24 genome while the people in group "B" showed 13.33% same positive reactions. This significant difference between exposed and nonexposed people to the positive equine reservoirs may imply the possible role of contact contamination in the epidemiology of BDV in IRAN. Conducting Western immune blotting technique on the same serum samples indicated the presence of serum antibody against BDV-P24 KDa protein in 23.33% in group "A", while the same positive reaction was 6.66% in group "B". The antibody against BDV-P40 Kda protein was detected in 46% of the people in group "A", while the same positive reaction was 6.66% of the people in group "B".

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APA: Copy

RAD, M.A., & NAKAYA, T.. (2001). DETECTION OF BORNA DISEASE VIRAL ANTIGEN (BDV-P24) IN PBMCS AS WELL AS ANTIBODIES AGAINST BDV-P24 AND BDV-P40 PROTEINS OF SERUM SAMPLES. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), 2(2), 207-193. SID. https://sid.ir/paper/568221/en

Vancouver: Copy

RAD M.A., NAKAYA T.. DETECTION OF BORNA DISEASE VIRAL ANTIGEN (BDV-P24) IN PBMCS AS WELL AS ANTIBODIES AGAINST BDV-P24 AND BDV-P40 PROTEINS OF SERUM SAMPLES. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR)[Internet]. 2001;2(2):207-193. Available from: https://sid.ir/paper/568221/en

IEEE: Copy

M.A. RAD, and T. NAKAYA, “DETECTION OF BORNA DISEASE VIRAL ANTIGEN (BDV-P24) IN PBMCS AS WELL AS ANTIBODIES AGAINST BDV-P24 AND BDV-P40 PROTEINS OF SERUM SAMPLES,” IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), vol. 2, no. 2, pp. 207–193, 2001, [Online]. Available: https://sid.ir/paper/568221/en

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