DETERMINING OF POLYMORPHISM OF FECB GENE IN LORY-BAKHTEYARIAN AND ARABIAN SHEEP OF KHOUZESTNIAN PROVINCE BY PCR-RFLP

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MOHAMMADI GH.A.; BEYGI NASIRI M.T.; AALIMOHAMMADI M.; MIRZADEH KH.; FAYAZI J.

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Paper Information

Journal: SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL Year:2009 | Volume:5 | Issue:3 (24) Page(s): 54-59

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Title

DETERMINING OF POLYMORPHISM OF FECB GENE IN LORY-BAKHTEYARIAN AND ARABIAN SHEEP OF KHOUZESTNIAN PROVINCE BY PCR-RFLP

Author(s)

Keywords

FECB MUTATION

FECUNDITY GENE

PCR-RFLP

LORY-BAKHTEYARI AND ARABIAN SHEEP

KHOZESTAN

Abstract

Using genetical markers is one of the best methods to evaluate animal improvement. It is reported that Booroola gene has additive for litter size and ovulation rate in sheep. In this study 125 blood samples were collected from Lory-Bakhteyarian (80 sheep) and Arabian sheep (45 sheep). DNA of blood samples was extracted by modified salting out method. The DNA was evaluated by spectrophotometer and agarose gel. Site of mutation was amplified using specific primers and PCR product (190 bp bands) was determined by agarose gel electrophoresis, and then the products PCR were digested with AvaIl enzyme. The homozygous carrier sheep with FECB MUTATION (BB) had a 160 bp and 30 bp bands, the noncarriers (++) had a 190 bp bands, whereas heterozygotes (B+) had 160, 30 and 190 bp bands. Results show no polymorphism for FecB gene in KHOZESTANian sheep. All of the sheep were wild type (++), so mutation of FecB gene was not the cause of prolificacy in KHOZESTANian sheep. Further research is required to evaluate FECUNDITY GENE and genotyping of KHOZESTANian sheep.

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APA: Copy

MOHAMMADI, GH.A., BEYGI NASIRI, M.T., AALIMOHAMMADI, M., MIRZADEH, KH., & FAYAZI, J.. (2009). DETERMINING OF POLYMORPHISM OF FECB GENE IN LORY-BAKHTEYARIAN AND ARABIAN SHEEP OF KHOUZESTNIAN PROVINCE BY PCR-RFLP. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 5(3 (24)), 54-59. SID. https://sid.ir/paper/569045/en

Vancouver: Copy

MOHAMMADI GH.A., BEYGI NASIRI M.T., AALIMOHAMMADI M., MIRZADEH KH., FAYAZI J.. DETERMINING OF POLYMORPHISM OF FECB GENE IN LORY-BAKHTEYARIAN AND ARABIAN SHEEP OF KHOUZESTNIAN PROVINCE BY PCR-RFLP. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2009;5(3 (24)):54-59. Available from: https://sid.ir/paper/569045/en

IEEE: Copy

GH.A. MOHAMMADI, M.T. BEYGI NASIRI, M. AALIMOHAMMADI, KH. MIRZADEH, and J. FAYAZI, “DETERMINING OF POLYMORPHISM OF FECB GENE IN LORY-BAKHTEYARIAN AND ARABIAN SHEEP OF KHOUZESTNIAN PROVINCE BY PCR-RFLP,” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 5, no. 3 (24), pp. 54–59, 2009, [Online]. Available: https://sid.ir/paper/569045/en

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