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Information Journal Paper

Title

EFFECT OF SALINITY ON CHANGES IN PROTEIN PROFILE IN SEEDLINGS OF WHEAT (TRITICUM AESTIVUM L.) CV. ROSHAN

Pages

  684-696

Abstract

 The effect of salinity on changes of protein profile in seedlings of wheat cv. Roshan was evaluated in a pot experiment in faculty of Agriculture, University of Tehran, in 2009. Seedlings of wheat cv. Roshan were exposed to 200 mM salinity for seventeen days. A proteomics technique was used to study proteins of leaf. Proteins of the leaves were extracted by TCA-acetone, and separated by two-dimensional gel electrophoresis at pH 4-7. Two hundred repeatable spots were identified and statistically analyzed. Twenty three spots were identified using MALDI TOF-TOF mass spectrometry. Expressions of nineteen spots increased and expressions of four spots decreased. Some identified proteins such as glutamine synthetase, ascorbate peroxidase, Cu/Zn superoxide dismutase and rubisco activase were up-regulated and other proteins like Oxygen-evolving enhancer protein and fructose-bisphosphate aldolase were down-regulated. Identified proteins are involved in regulation of carbohydrate, nitrogen and energy metabolism and eliminating of ROS. In this study, we recognized some proteins involved in salt tolerance at seedling stage in the cv. Roshan.

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    APA: Copy

    MALEKI, M., NAGHAVI, M.R., ALIZADEH, H., POUSTINI, K., & ABD MISHANI, S.. (2012). EFFECT OF SALINITY ON CHANGES IN PROTEIN PROFILE IN SEEDLINGS OF WHEAT (TRITICUM AESTIVUM L.) CV. ROSHAN. IRANIAN JOURNAL OF CROP SCIENCES, 13(4 (52)), 684-696. SID. https://sid.ir/paper/57305/en

    Vancouver: Copy

    MALEKI M., NAGHAVI M.R., ALIZADEH H., POUSTINI K., ABD MISHANI S.. EFFECT OF SALINITY ON CHANGES IN PROTEIN PROFILE IN SEEDLINGS OF WHEAT (TRITICUM AESTIVUM L.) CV. ROSHAN. IRANIAN JOURNAL OF CROP SCIENCES[Internet]. 2012;13(4 (52)):684-696. Available from: https://sid.ir/paper/57305/en

    IEEE: Copy

    M. MALEKI, M.R. NAGHAVI, H. ALIZADEH, K. POUSTINI, and S. ABD MISHANI, “EFFECT OF SALINITY ON CHANGES IN PROTEIN PROFILE IN SEEDLINGS OF WHEAT (TRITICUM AESTIVUM L.) CV. ROSHAN,” IRANIAN JOURNAL OF CROP SCIENCES, vol. 13, no. 4 (52), pp. 684–696, 2012, [Online]. Available: https://sid.ir/paper/57305/en

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