APPLICATION OF THE RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) TECHNIQUE, AS A DNA MARKER FOR DETECTION OF POLYMORPHISM AMONG ITALIAN DURUM WHEAT CULTIVARS (TRITICUM TURGIDUM L. SPP. DURUM).

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BEHNAM BABAK; YAZDI SAMADI B.; ABD MISHANI C.; TALEI A.; SHAHNEZHAT BOUSHEHRI A.A.

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Journal: Iranian Journal of Crop Sciences Year:2000 | Volume:2 | Issue:2 Page(s): 29-39

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Title

APPLICATION OF THE RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) TECHNIQUE, AS A DNA MARKER FOR DETECTION OF POLYMORPHISM AMONG ITALIAN DURUM WHEAT CULTIVARS (TRITICUM TURGIDUM L. SPP. DURUM).

Author(s)

Keywords

POLYMORPHISMQ2

RAPD-PCRQ2

NEI'S SIMILARITY COEFFICIENTQ4

DURUM WHEATQ4

Abstract

Development of a linkage map of cultivated wheats using conventional molecular markers has lagged behind, because of the large genome size and limited levels of genetic POLYMORPHISMs. Recently RAPD (PCR) marker have been suggested to provide an alternative to visualize more POLYMORPHISM. In this research the data obtained by using RAPD analysis of 10 arbitrary primers (10 mers) in 8 DURUM WHEAT and 9 advanced line derived from a diallel cross between two cultivars. A total 297 and 665 RAPD fragments were generated in polyacrylamide and agarose gels respectively. Amplification reactions resulted in fragments ranging in length between 300 and 3000 bps. Bands were scored in binary matrix and this matrix was used for construction the distance matrix based on Nei’s similarity coefficient. The amplified products were treated as independent characters to generate a phenogram using cluster analysis and cultivars classified in 5 groups for agarose gels and 4 groups for polyacrylamide gels. In both agarose and polyacrylamide gels, X2 analysis showed that, there are differences between varieties and primers regarding band generation. We also detected specific bands that existed in advanced lines. The results showed that RAPD markers are suitable for studying DNA POLYMORPHISM.

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References

استفاده از نشانگرهای مولکولی RAPD در بررسی گوناگونی ژنتیکی گونه های Triticum boeoticum و T.urartu

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APA: Copy

BEHNAM, BABAK, YAZDI SAMADI, B., ABD MISHANI, C., TALEI, A., & SHAHNEZHAT BOUSHEHRI, A.A.. (2000). APPLICATION OF THE RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) TECHNIQUE, AS A DNA MARKER FOR DETECTION OF POLYMORPHISM AMONG ITALIAN DURUM WHEAT CULTIVARS (TRITICUM TURGIDUM L. SPP. DURUM).. IRANIAN JOURNAL OF CROP SCIENCES, 2(2), 29-39. SID. https://sid.ir/paper/57355/en

Vancouver: Copy

BEHNAM BABAK, YAZDI SAMADI B., ABD MISHANI C., TALEI A., SHAHNEZHAT BOUSHEHRI A.A.. APPLICATION OF THE RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) TECHNIQUE, AS A DNA MARKER FOR DETECTION OF POLYMORPHISM AMONG ITALIAN DURUM WHEAT CULTIVARS (TRITICUM TURGIDUM L. SPP. DURUM).. IRANIAN JOURNAL OF CROP SCIENCES[Internet]. 2000;2(2):29-39. Available from: https://sid.ir/paper/57355/en

IEEE: Copy

BABAK BEHNAM, B. YAZDI SAMADI, C. ABD MISHANI, A. TALEI, and A.A. SHAHNEZHAT BOUSHEHRI, “APPLICATION OF THE RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) TECHNIQUE, AS A DNA MARKER FOR DETECTION OF POLYMORPHISM AMONG ITALIAN DURUM WHEAT CULTIVARS (TRITICUM TURGIDUM L. SPP. DURUM).,” IRANIAN JOURNAL OF CROP SCIENCES, vol. 2, no. 2, pp. 29–39, 2000, [Online]. Available: https://sid.ir/paper/57355/en

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