INCREASING OF IN VITRO MULTIPLICATION RATE OF SUGAR BEET USING PERIODICAL BIOREACTOR

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ROUZBEH F.; MESBAH M.; DAVOUDI D.

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Journal Paper

Paper Information

Journal: Journal of Sugar Beet Year:2005 | Volume:20 | Issue:2 Page(s): 113-125

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Information Journal Paper

Title

INCREASING OF IN VITRO MULTIPLICATION RATE OF SUGAR BEET USING PERIODICAL BIOREACTOR

Author(s)

ROUZBEH F. | MESBAH M. | DAVOUDI D. | Issue Writer Certificate

Keywords

SUGAR BEETQ4

PERIODICAL BIOREACTORQ4

MICROPROPAGATIONQ3

ECONOMICAL METHODQ4

Abstract

Selected genotypes of SUGAR BEET are often maintained by conventional propagation, but much faster rates of multiplication can be now obtained through MICROPROPAGATION. On the other hand, plant MICROPROPAGATION using bioreactors can decrease the cost of production and makes it an ECONOMICAL METHOD. In this research, the possibility of using bioreactor and optimization of the tissue culture procedure in SUGAR BEET MICROPROPAGATION was studied. A simple PERIODICAL BIOREACTOR was made by using a timer, air pump, microfilters (0.2 μ), and air and medium autoclaveable transfer pipes. In vitro stock plantlets from seeds and liquid MS basal medium supplemented with 1.5 mg.l-1 BAP, 0.2 mg.l-1 NAA, 0.5 mg.l-1 GA3, and 3% sucrose were used for inoculation and multiplication in the bioreactors. Periodical nutrition of the explants established for 10 minutes every 6 hours. The same basal medium with 3 mg.l-1 IBA was used for root induction. Bioreactor system was compared with routine in vitro procedure in tissue culture laboratory. Results showed that the bioreactor system multiplication rate, number of shoots, and biomass production was significantly different from routine in vitro culture in 100 ml jars. Rooting percentage in bioreactor was very low and needs more study to optimization.

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References

PRODUCTION OF HEALTHY MICROTUBERS OF POTATO IN AFTER NATIVE BIOVEACTOR SYSTEM

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APA: Copy

ROUZBEH, F., MESBAH, M., & DAVOUDI, D.. (2005). INCREASING OF IN VITRO MULTIPLICATION RATE OF SUGAR BEET USING PERIODICAL BIOREACTOR. JOURNAL OF SUGAR BEET, 20(2), 113-125. SID. https://sid.ir/paper/57612/en

Vancouver: Copy

ROUZBEH F., MESBAH M., DAVOUDI D.. INCREASING OF IN VITRO MULTIPLICATION RATE OF SUGAR BEET USING PERIODICAL BIOREACTOR. JOURNAL OF SUGAR BEET[Internet]. 2005;20(2):113-125. Available from: https://sid.ir/paper/57612/en

IEEE: Copy

F. ROUZBEH, M. MESBAH, and D. DAVOUDI, “INCREASING OF IN VITRO MULTIPLICATION RATE OF SUGAR BEET USING PERIODICAL BIOREACTOR,” JOURNAL OF SUGAR BEET, vol. 20, no. 2, pp. 113–125, 2005, [Online]. Available: https://sid.ir/paper/57612/en

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