A STUDY ON GENETIC DIVERSITY OF IRANIAN POPULATIONS OF ERYSIPHE BETAE(VANHA) WELTZIEN CAUSAL AGENT OF SUGAR BEET POWDERY MILDEW USING RDNA-RFLP METHOD

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SHEYKH ALESLAMI S.M.; OKHOVAT M.; JAVAN NIKKHAH M.; HEJAROUD GH.A.; SHARIFI TEHRANI A.; SEIDEL M.

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Journal: Journal of Sugar Beet Year:2005 | Volume:20 | Issue:2 Page(s): 149-159

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Title

A STUDY ON GENETIC DIVERSITY OF IRANIAN POPULATIONS OF ERYSIPHE BETAE(VANHA) WELTZIEN CAUSAL AGENT OF SUGAR BEET POWDERY MILDEW USING RDNA-RFLP METHOD

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Keywords

GENETIC DIVERSITYQ3

IRANIAN POPULATIONSQ4

POWDERY MILDEWQ3

RDNA-RFLPQ3

SUGAR BEETQ4

ERYSIPHE BETAEQ4

Abstract

A total of 105 isolates of ERYSIPHE BETAE from different regions of Iran were investigated. For probable polymorphism of ITS-5.8S regions using PCR-based RFLP, the mentioned fragments were amplified via PCR by ITS1 and ITS4 primers and subjected to 1.5% agarose gel electrophoresis. PCR products were digested by HaeIII, AluI, TaqI, HindIII, CfoI, EcoRI, MspI, SacI and RsaI REs and the digestion products were resolved on 7.5% polyacrylamide. PCR product of all isolates was only one 645 bp fragment. Among nine REs tested AluI, CfoI, MspI and HaeIII had recognition sequences on PCR products. Because of unstability, results for AluI were ignored.Digestion products for CfoI were 280, 185 and 180bp, for MspI, 325, 160, 120 and 40bp and those of HaeIII, 325, 180, 75, 40 and 30bp fragments for all isolates. Electrophoretic patterns of PCR products and digested products were completely similar,thus no polymorphism was discernible between 105 isolates. Based on the results,Iranian populations of E.betae seems to be highly uniform.

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APA: Copy

SHEYKH ALESLAMI, S.M., OKHOVAT, M., JAVAN NIKKHAH, M., HEJAROUD, GH.A., SHARIFI TEHRANI, A., & SEIDEL, M.. (2005). A STUDY ON GENETIC DIVERSITY OF IRANIAN POPULATIONS OF ERYSIPHE BETAE(VANHA) WELTZIEN CAUSAL AGENT OF SUGAR BEET POWDERY MILDEW USING RDNA-RFLP METHOD. JOURNAL OF SUGAR BEET, 20(2), 149-159. SID. https://sid.ir/paper/57615/en

Vancouver: Copy

SHEYKH ALESLAMI S.M., OKHOVAT M., JAVAN NIKKHAH M., HEJAROUD GH.A., SHARIFI TEHRANI A., SEIDEL M.. A STUDY ON GENETIC DIVERSITY OF IRANIAN POPULATIONS OF ERYSIPHE BETAE(VANHA) WELTZIEN CAUSAL AGENT OF SUGAR BEET POWDERY MILDEW USING RDNA-RFLP METHOD. JOURNAL OF SUGAR BEET[Internet]. 2005;20(2):149-159. Available from: https://sid.ir/paper/57615/en

IEEE: Copy

S.M. SHEYKH ALESLAMI, M. OKHOVAT, M. JAVAN NIKKHAH, GH.A. HEJAROUD, A. SHARIFI TEHRANI, and M. SEIDEL, “A STUDY ON GENETIC DIVERSITY OF IRANIAN POPULATIONS OF ERYSIPHE BETAE(VANHA) WELTZIEN CAUSAL AGENT OF SUGAR BEET POWDERY MILDEW USING RDNA-RFLP METHOD,” JOURNAL OF SUGAR BEET, vol. 20, no. 2, pp. 149–159, 2005, [Online]. Available: https://sid.ir/paper/57615/en

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