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Information Journal Paper

Title

EFFECT OF VARIOUS LEVELS OF CATALASE ANTIOXIDANT IN SEMEN EXTENDERS ON LIPID PEROXIDATION AND SEMEN QUALITY AFTER THE FREEZE-THAWING BULL SEMEN

Pages

  218-221

Abstract

 The objective of this study was to evaluate effect of different concentrations of CATALASE in two extenders on motility, viability and LIPID PEROXIDATION BULL spermatozoa during semen freezing process. Thirty ejaculates collected from ten Holstein BULLs were pooled and evaluated at 37oC. Pool ejaculated was split into two main experimental groups, 1 and 2. In experiment 1, specimen was diluted to a final concentration of 30×106 spermatozoa with citrate-egg yolk and in experiment 2; specimen was diluted with tris-egg yolk extender to the same concentration. In both experiments diluted semen was divided into three aliquots, including a control and two test groups. Each aliquot was rediluted with an equal volume of extender either without (control) or with one of the antioxidants contained one of the following antioxidants: CATALASE (CAT; 100 IU mL-1) CATALASE (CAT; 200 IU mL-1) and control group. No significant differences were observed in sperm viability and motility following addition of CATALASE enzyme at concentration of 100 IU mL-1 and 200 IU mL-1 to citrate-egg yolk extender. But the highest sperm viability was achieved by addition of 100 IU mL-1 and 200 IU mL-1 CATALASE to tris-egg yolk semen extender compared with the control group (P<0.05). Malondialdehyde levels did not change with addition of CATALASE in both extenders compared with the control group. The obtained results provide a new approach to the cryopreservation of BULL semen, and could positively contribute to intensive cattle production.

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    • Cite

    APA: Copy

    ASADPOUR, REZA, JAFARI, RAZI, & TAYEFI NASRABADI, HOSSEIN. (2011). EFFECT OF VARIOUS LEVELS OF CATALASE ANTIOXIDANT IN SEMEN EXTENDERS ON LIPID PEROXIDATION AND SEMEN QUALITY AFTER THE FREEZE-THAWING BULL SEMEN. VETERINARY RESEARCH FORUM, 2(4), 218-221. SID. https://sid.ir/paper/591263/en

    Vancouver: Copy

    ASADPOUR REZA, JAFARI RAZI, TAYEFI NASRABADI HOSSEIN. EFFECT OF VARIOUS LEVELS OF CATALASE ANTIOXIDANT IN SEMEN EXTENDERS ON LIPID PEROXIDATION AND SEMEN QUALITY AFTER THE FREEZE-THAWING BULL SEMEN. VETERINARY RESEARCH FORUM[Internet]. 2011;2(4):218-221. Available from: https://sid.ir/paper/591263/en

    IEEE: Copy

    REZA ASADPOUR, RAZI JAFARI, and HOSSEIN TAYEFI NASRABADI, “EFFECT OF VARIOUS LEVELS OF CATALASE ANTIOXIDANT IN SEMEN EXTENDERS ON LIPID PEROXIDATION AND SEMEN QUALITY AFTER THE FREEZE-THAWING BULL SEMEN,” VETERINARY RESEARCH FORUM, vol. 2, no. 4, pp. 218–221, 2011, [Online]. Available: https://sid.ir/paper/591263/en

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