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Information Journal Paper

Title

DISCRIMINATION AND PRELIMINARY SELECTION OF SELF-COMPATIBLE PROGENIES AMONG CONTROLLED CROSSES IN ALMOND USING SF SPECIFIC PRIMER

Pages

  47-54

Abstract

 Self-incompatibility in ALMOND andPrunus species is an important trait that prevents self-fertilization. Selfincompatibility in ALMOND is controlled game tophytically by the multiallelic S-locus. Present study was done in order to identification and preliminary selection of self-compatible progenies resulted from controlled crosses in ALMOND using specific primer SfF/SfR. Some important morphological traits of parental crosses were evaluated using ALMOND descriptor. Also, progenies (F1) of five crosses inculding; A (Tuono × 101 Genotype), B (Supernova × 101 Genotype), C (Genco × Shahrood 21), D (Tuono × Shahrood 12) and E (Tuono × shahrood 17) were tested using PCR in order to DNA amplification and self-compatibility evaluation. The result of PCR method found that using SfF/SfR pair primer, self-compatible progenies showed 449 bp bands, while this band not observed in self-incompatible progenies. In addition, all self- incompatible progenies appeared no S1 allele at any condition. According to Chi-square test, ratios of self-compatible to self-incompatible progenies were to Mendelian principles. It can be suggested that PCR method of this research has high potential in order to distinguish self-compatible and incompatible genotypes.

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    APA: Copy

    SHAHMORADI, M., RASOULI, M., FOOTUHI GHAZVINI, R., IMANI, A., & HAMIDOGLI, Y.. (2012). DISCRIMINATION AND PRELIMINARY SELECTION OF SELF-COMPATIBLE PROGENIES AMONG CONTROLLED CROSSES IN ALMOND USING SF SPECIFIC PRIMER. JOURNAL OF NUTS (INTERNATIONAL JOURNAL OF NUTS AND RELATED SCIENCES), 3(3), 47-54. SID. https://sid.ir/paper/616640/en

    Vancouver: Copy

    SHAHMORADI M., RASOULI M., FOOTUHI GHAZVINI R., IMANI A., HAMIDOGLI Y.. DISCRIMINATION AND PRELIMINARY SELECTION OF SELF-COMPATIBLE PROGENIES AMONG CONTROLLED CROSSES IN ALMOND USING SF SPECIFIC PRIMER. JOURNAL OF NUTS (INTERNATIONAL JOURNAL OF NUTS AND RELATED SCIENCES)[Internet]. 2012;3(3):47-54. Available from: https://sid.ir/paper/616640/en

    IEEE: Copy

    M. SHAHMORADI, M. RASOULI, R. FOOTUHI GHAZVINI, A. IMANI, and Y. HAMIDOGLI, “DISCRIMINATION AND PRELIMINARY SELECTION OF SELF-COMPATIBLE PROGENIES AMONG CONTROLLED CROSSES IN ALMOND USING SF SPECIFIC PRIMER,” JOURNAL OF NUTS (INTERNATIONAL JOURNAL OF NUTS AND RELATED SCIENCES), vol. 3, no. 3, pp. 47–54, 2012, [Online]. Available: https://sid.ir/paper/616640/en

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