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Information Journal Paper

Title

IN VITRO MICRO PROPAGATION, CALLUS INDUCTION AND SHOOT REGENERATION IN SAFFLOWER L. CV. LESAF

Pages

  999-1004

Abstract

 The present study was conducted to induce callus from different in vitro grown seedling explants of shoot, root and leaf segments in order to develop shoot buds and rooting from callus. The sterilized seeds were cultured on Murashige and Skoog medium, and then the explants were cultured from seedling and transferred to an MS medium supplemented with different concentrations of BAP, KINETIN, NAA, and 2, 4-D growth regulator hormones. Among all explants, the leaf explants should produce significant CALLUS INDUCTION (97.79 %) on MS +1.0 mgl-1 BAP medium. After 4 weeks, the obtained calluses were transferred to a new regeneration media with different concentrations of BAP and NAA and then the calluses were sub cultured on the same medium every 2 weeks. Overall, the maximum regeneration frequency was obtained from MS medium containing 0.1 mgl-1 NAA and 1.0 mgl-1 BAP.

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    Cite

    APA: Copy

    GHASEMPOUR, HAMIDREZA, SOHEILIKHAH, ZHALEH, ZEBARJADI, ALI REZA, GHASEMPOUR, SABA, & KARIMI, NASER. (2014). IN VITRO MICRO PROPAGATION, CALLUS INDUCTION AND SHOOT REGENERATION IN SAFFLOWER L. CV. LESAF. IRANIAN JOURNAL OF PLANT PHYSIOLOGY, 4(2), 999-1004. SID. https://sid.ir/paper/616643/en

    Vancouver: Copy

    GHASEMPOUR HAMIDREZA, SOHEILIKHAH ZHALEH, ZEBARJADI ALI REZA, GHASEMPOUR SABA, KARIMI NASER. IN VITRO MICRO PROPAGATION, CALLUS INDUCTION AND SHOOT REGENERATION IN SAFFLOWER L. CV. LESAF. IRANIAN JOURNAL OF PLANT PHYSIOLOGY[Internet]. 2014;4(2):999-1004. Available from: https://sid.ir/paper/616643/en

    IEEE: Copy

    HAMIDREZA GHASEMPOUR, ZHALEH SOHEILIKHAH, ALI REZA ZEBARJADI, SABA GHASEMPOUR, and NASER KARIMI, “IN VITRO MICRO PROPAGATION, CALLUS INDUCTION AND SHOOT REGENERATION IN SAFFLOWER L. CV. LESAF,” IRANIAN JOURNAL OF PLANT PHYSIOLOGY, vol. 4, no. 2, pp. 999–1004, 2014, [Online]. Available: https://sid.ir/paper/616643/en

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