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Information Journal Paper

Title

DEVELOPMENT OF ANTIBODY-BASED MICROARRAY ASSAY FOR QUANTITATIVE DETECTION OF AFLATOXIN B1

Pages

  135-142

Keywords

AFLATOXIN B1 

Abstract

 BACKGROUND: Aflatoxin B1 (AFB1) is a toxic metabolite produced by Aspergillus species that contaminates a wide range of agricultural products.OBJECTIVES: This study was designed to develop a rapid and highly sensitive IMMUNOASSAY method in MICROARRAY format for quantitative detection of AFB1 to evaluate the potential of MICROARRAY platform for high-throughput screening, which can be beneficial in food and feed industry.METHODS: Following successful OPTIMIZATION, using an indirect competitive IMMUNOASSAY in DOT BLOT format, AFB1-bovine serum albumin (AFB1-BSA) conjugate was contact-printed onto 16 isolated subarrays on multi-pad nitrocellulose coated slides; subsequently used in competitive binding assays.RESULTS: Using the aforementioned assay, AFB1 was determined from 15 pg/g to 3.04 ng/g working range with detection limit (LOD) of 1 pg/g. To evaluate assay performance in real food matrices, the authors spiked wheat samples with different concentration of AFB1. After extraction, working ranges of 0.11-4.15 ng/g with detection limit of 30pg/g was determined. Good recoveries (94±9%) were obtained, demonstrating accurate detection of AFB1 concentrations in wheat samples. Assay procedure completed in 3 hours.CONCLUSIONS: The results indicated that the proposed developed assay in MICROARRAY format could be used for rapid and sensitive detection of AFB1in wheat samples.

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    APA: Copy

    BEIZAEI, A., KAMKAR, A., & MISAGHI, A.. (2014). DEVELOPMENT OF ANTIBODY-BASED MICROARRAY ASSAY FOR QUANTITATIVE DETECTION OF AFLATOXIN B1. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), 8(2), 135-142. SID. https://sid.ir/paper/629366/en

    Vancouver: Copy

    BEIZAEI A., KAMKAR A., MISAGHI A.. DEVELOPMENT OF ANTIBODY-BASED MICROARRAY ASSAY FOR QUANTITATIVE DETECTION OF AFLATOXIN B1. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH)[Internet]. 2014;8(2):135-142. Available from: https://sid.ir/paper/629366/en

    IEEE: Copy

    A. BEIZAEI, A. KAMKAR, and A. MISAGHI, “DEVELOPMENT OF ANTIBODY-BASED MICROARRAY ASSAY FOR QUANTITATIVE DETECTION OF AFLATOXIN B1,” IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), vol. 8, no. 2, pp. 135–142, 2014, [Online]. Available: https://sid.ir/paper/629366/en

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