FREQUENCY OF β-LACTAMASE PRODUCING ISOLATES OF ESCHERICHIA COLI AND THEIR DIVERSITY IN ENZYME ACTIVITIES AMONG THE RESISTANCE ISOLATES FROM PATIENTS WITH DIARRHEA AND NOSOCOMIAL INFECTIONS IN TEHRAN, IRAN

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RAHIMI MOHAMMAD; TAJBAKHSH MERCEDEH; RAZAGHI MARYAM; TAJEDDIN ELAHE; ALEBOUYEH MASOUD; RAJABI BAZL MASOUMEH; ZALI MOHAMMAD REZA

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Journal: AMIRKABIR Year:2014 | Volume:15 | Issue:2 (50) Page(s): 197-205

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Title

FREQUENCY OF β-LACTAMASE PRODUCING ISOLATES OF ESCHERICHIA COLI AND THEIR DIVERSITY IN ENZYME ACTIVITIES AMONG THE RESISTANCE ISOLATES FROM PATIENTS WITH DIARRHEA AND NOSOCOMIAL INFECTIONS IN TEHRAN, IRAN

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Keywords

BETA LACTAMASE

ESCHERICHIA COLI

CROSS INFECTION

DRUG RESISTANCE

BACTERIAL DRUG RESISTANCE

IRAN

Abstract

Introduction: The risky multidrug resistance phenotypes could be spread among strains of ESCHERICHIA COLI (E. coli). The resistance against b-lactam drugs could emerge through the production of different enzymes with different spectrum of activities. This study was planned to determine the activity of b- lactamases extracted from different clinical isolates of E. coli and their relatedness with resistance phenotype and plasmid patterns.Materials and Methods: Antimicrobial susceptibility of E. coli isolates from different clinical samples to 10 b-lactam antibiotics were studied by disk diffusion method and minimal inhibitory concentration (MIC) of the resistant isolates were determined against cefotaxime by agar dilution method. Iodometry assay was used to detect b-lactamase activity of periplasmic fractions of the protein extracts in the presence of cefotaxime. Plasmid transmissibility of the resistance phenotype into E. coli HB101 was finally studied by CaCl2 transformation method.Results: Results of this research showed existence of high plenty of multidrug resistance phenotypes (21%) among these isolates. The study-generated antibiogram showed that the lowest percent resistance antibiotics for all the isolates were imipenem (11%) and ceftazidime (15.52%). This resistance phenotype was transferable by plasmids in strain E. coli HB101. Analysis of the b-lactamase activities showed a variation among them in ranges of 0,007 upto 0,065 units. No significant relationship was observed between the level of MIC (<4 to 1024 mg/ml) and the amounts of enzyme activities.Conclusion: These results indicated that type of the produced enzymes and also their specificities for b-lactam drugs, but not the amount of their total enzyme activities, do determine the level of their resistance phenotypes and MIC levels. Presence of the high levels of MIC in some isolates with very low enzyme activities suggested the existence of other mechanisms of DRUG RESISTANCE among them. Identification of antibiotics with higher affinity for these b-lactamases will provide valuable information about fighting against these bacteria in severe infections.

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APA: Copy

RAHIMI, MOHAMMAD, TAJBAKHSH, MERCEDEH, RAZAGHI, MARYAM, TAJEDDIN, ELAHE, ALEBOUYEH, MASOUD, RAJABI BAZL, MASOUMEH, & ZALI, MOHAMMAD REZA. (2014). FREQUENCY OF β-LACTAMASE PRODUCING ISOLATES OF ESCHERICHIA COLI AND THEIR DIVERSITY IN ENZYME ACTIVITIES AMONG THE RESISTANCE ISOLATES FROM PATIENTS WITH DIARRHEA AND NOSOCOMIAL INFECTIONS IN TEHRAN, IRAN. AMIRKABIR, 15(2 (50)), 197-205. SID. https://sid.ir/paper/637754/en

Vancouver: Copy

RAHIMI MOHAMMAD, TAJBAKHSH MERCEDEH, RAZAGHI MARYAM, TAJEDDIN ELAHE, ALEBOUYEH MASOUD, RAJABI BAZL MASOUMEH, ZALI MOHAMMAD REZA. FREQUENCY OF β-LACTAMASE PRODUCING ISOLATES OF ESCHERICHIA COLI AND THEIR DIVERSITY IN ENZYME ACTIVITIES AMONG THE RESISTANCE ISOLATES FROM PATIENTS WITH DIARRHEA AND NOSOCOMIAL INFECTIONS IN TEHRAN, IRAN. AMIRKABIR[Internet]. 2014;15(2 (50)):197-205. Available from: https://sid.ir/paper/637754/en

IEEE: Copy

MOHAMMAD RAHIMI, MERCEDEH TAJBAKHSH, MARYAM RAZAGHI, ELAHE TAJEDDIN, MASOUD ALEBOUYEH, MASOUMEH RAJABI BAZL, and MOHAMMAD REZA ZALI, “FREQUENCY OF β-LACTAMASE PRODUCING ISOLATES OF ESCHERICHIA COLI AND THEIR DIVERSITY IN ENZYME ACTIVITIES AMONG THE RESISTANCE ISOLATES FROM PATIENTS WITH DIARRHEA AND NOSOCOMIAL INFECTIONS IN TEHRAN, IRAN,” AMIRKABIR, vol. 15, no. 2 (50), pp. 197–205, 2014, [Online]. Available: https://sid.ir/paper/637754/en

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