Biochemical properties of digestive carbohydrases and proteases of cereal leaf beetle, Lema melanopa (Coleoptera: Chrysomelidae)

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Sharifi Mahboobeh; Ghadmyari Mohammad; Memarizadeh Nargess

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Journal Paper

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Journal: Journal of Entomological Society of Iran Year:2020 | Volume:40 | Issue:3 Page(s): 323-338

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Information Journal Paper

Title

Biochemical properties of digestive carbohydrases and proteases of cereal leaf beetle, Lema melanopa (Coleoptera: Chrysomelidae)

Author(s)

Sharifi Mahboobeh | Ghadmyari Mohammad | Memarizadeh Nargess | Issue Writer Certificate

Keywords

alpha

amylase

and β

glucosidase

and β

galactosidase

protease inhibitor

prote

ases

cereal leaf beetle

Abstract

During recent decade, Lema melanopa L. has become one of the most important pests of wheat in Golstan province. Both adults and larvae of this pest feed on the leaves of most cereals and of course wheat and cause severe damage. Due to the lack of information about the physiology of this pest, in the present study, the biochemical properties of alpha amylase, glucosidase, galactosidase and gastro-intestinal proteases from adult stage of this pest were investigated. The results showed that the highest level of optimal enzyme activity was observed in the range of 4 to 6 and the optimum temperature of 35 ° C. Electrophoresis showed two isoforms of alpha-amylase in the gut of the insect. The maximum activity of alpha and beta glucosidase enzymes and alpha and beta galactosidase of the gut was ob-tained in acidity 5 and in the temperature range of 35 to 45° C, respectively. The highest general pro-tease activity was shown among gut proteases at pH 6 and at 45° C. Obtained results regarding the effect of enzymatic inhibitors including EDTA, TLCK, TPCK, PMSF, sodium Iodo Acetate and Io-doacetic acid protease activity showed that cysteine proteinase inhibitors had the greatest inhibitory effect on enzyme activity. Therefore, it can be concluded that this group of proteases is dominant in the gut of cereal leaf beetle. By determining the biochemical properties of important gut enzymes of pest, compounds can be synthesized that are more efficient and reduce the consumption of insecti-cides and reduce the amount of damage to the environment.

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APA: Copy

Sharifi, Mahboobeh, Ghadmyari, Mohammad, & Memarizadeh, Nargess. (2020). Biochemical properties of digestive carbohydrases and proteases of cereal leaf beetle, Lema melanopa (Coleoptera: Chrysomelidae). JOURNAL OF ENTOMOLOGICAL SOCIETY OF IRAN (JESI), 40(3 ), 323-338. SID. https://sid.ir/paper/659300/en

Vancouver: Copy

Sharifi Mahboobeh, Ghadmyari Mohammad, Memarizadeh Nargess. Biochemical properties of digestive carbohydrases and proteases of cereal leaf beetle, Lema melanopa (Coleoptera: Chrysomelidae). JOURNAL OF ENTOMOLOGICAL SOCIETY OF IRAN (JESI)[Internet]. 2020;40(3 ):323-338. Available from: https://sid.ir/paper/659300/en

IEEE: Copy

Mahboobeh Sharifi, Mohammad Ghadmyari, and Nargess Memarizadeh, “Biochemical properties of digestive carbohydrases and proteases of cereal leaf beetle, Lema melanopa (Coleoptera: Chrysomelidae),” JOURNAL OF ENTOMOLOGICAL SOCIETY OF IRAN (JESI), vol. 40, no. 3 , pp. 323–338, 2020, [Online]. Available: https://sid.ir/paper/659300/en

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