Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

308
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

0
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

CLONING AND LTP2 GENE EXPRESSION FALLAT WHEAT IN BEAN PLANT AND STUDY ITS ANTIMICROBIAL EFFECT

Pages

  0-0

Abstract

 lipid transfer protein are small peptides and rich in cysteine and based on the molecular weight divided into two groups LTP1 and LTP2. These proteins have different biological activities and are involved in plant defense mechanisms. By analyzing microarray data from different experiments indicated that LTP genes in biotic and abiotic stresses have increased or decreased expression. In this study, in order to investigate and determine the incidence and impact LTP genes in resistance wheat and barley to Fusarium graminearum, Puccinia, Ustilago, four microarray libraries with sensitive and resistant isolines from the NCBI database were taken and LTP genes expression pattern graph was drawn. And LTP gene expression pattern graph was plotted. The charts showed that different LTP genes in resistant and susceptible cultivars to the diseases have shown differences in expression. In result, LTP gene can be introduced as one of the factors contributing to the resistance. It was found that differences between genes expression patterns, in resistant and susceptible cultivars after infecting with the disease may have a major role in the mechanisms of disease resistance in wheat and barley. To construct the gene expression system LTP2, LTP2 gene isolated from Fallat wheat was transferred and to pBISN1-IN plasmid. The recombinant plasmid was cloned in E.coli strain DH5α. To investigate the expression system performance the recombinant plasmid was extracted and then transferred to Agrobacterium strain LBA4404 and then transferred to beans. This expression system were assessed in RNA levels and amount of active soluble protein in leaf injected with recombinant plasmid. Expression and function of the protein was confirmed and chimeric protein showed antibacterial effect on Staphylococcus aureus.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    ARIANPUR, P., MIRAKHORLI, N., & RAFIEI, F.. (2017). CLONING AND LTP2 GENE EXPRESSION FALLAT WHEAT IN BEAN PLANT AND STUDY ITS ANTIMICROBIAL EFFECT. MODERN GENETICS JOURNAL (MGJ), 11(4), 0-0. SID. https://sid.ir/paper/700188/en

    Vancouver: Copy

    ARIANPUR P., MIRAKHORLI N., RAFIEI F.. CLONING AND LTP2 GENE EXPRESSION FALLAT WHEAT IN BEAN PLANT AND STUDY ITS ANTIMICROBIAL EFFECT. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2017;11(4):0-0. Available from: https://sid.ir/paper/700188/en

    IEEE: Copy

    P. ARIANPUR, N. MIRAKHORLI, and F. RAFIEI, “CLONING AND LTP2 GENE EXPRESSION FALLAT WHEAT IN BEAN PLANT AND STUDY ITS ANTIMICROBIAL EFFECT,” MODERN GENETICS JOURNAL (MGJ), vol. 11, no. 4, pp. 0–0, 2017, [Online]. Available: https://sid.ir/paper/700188/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    مرکز اطلاعات علمی SID
    strs
    دانشگاه امام حسین
    بنیاد ملی بازیهای رایانه ای
    کلید پژوه
    ایران سرچ
    ایران سرچ
    File Not Exists.
    Move to top