Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

237
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

0
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

SURVEY GENOTYPING OF ANIMAL AND HUMAN KLEBSIELLA PNEUMONIAE ISOLATES USING ERIC-PCR AND EVALUATION OF ANTIBIOTIC SENSITIVITY PATTERN

Pages

  2469-2477

Abstract

KLEBSIELLA PNEUMONIAE, is an opportunistic pathogens and cause infections in humans and animals. Drug resistant K. pneumoniae is rising. Therefore, antimicrobial susceptibility testing before prescribing antibiotics, it seems necessary. The aim of present study was to survey typing of clinical and animal K. pneumoniae isolates and evaluation of antibiotic susceptibility. A total 100 clinical and animal K. pneumoniae isolates were collected from Babak city. Antibiotic susceptibility was performed with Kirby-Bauer method according to CLSI guidelines. Then, DNA genomic extraction was done using DNA kit and PCR amplification was performed with ERIC1 and ERIC2 primer. Our results were shown that all strains (100%) were resistant to the ampicillin and amikacin antibiotics. The most and least resistance belong to tetracycline (53 strains; 88.3%) and imipenem (8 isolates; 13.3%), respectively. The results of cluster analysis and drawing dendrogram based on genetic similarities for 100 isolates was separated to seventeen distinct groups. According to our finding indicated an increasing resistance to antibiotics amongst K. pneumoniae. Additionally, the ERIC sequences have a pair of games that contain highly reversed and central reps and are located in the outermost regions of the bacterial genome and have less complexity in determining the genetic diversity of all isolates, but the separation good at the strain level.

Cites

  • No record.
  • References

  • No record.
  • Cite

    APA: Copy

    ESTABRAGHI, E., ZAHRAEI SALEHI, T., AMINI, K., & JAMSHIDIAN, M.. (2018). SURVEY GENOTYPING OF ANIMAL AND HUMAN KLEBSIELLA PNEUMONIAE ISOLATES USING ERIC-PCR AND EVALUATION OF ANTIBIOTIC SENSITIVITY PATTERN. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, 15 (NEW)(2 (61) ), 2469-2477. SID. https://sid.ir/paper/714283/en

    Vancouver: Copy

    ESTABRAGHI E., ZAHRAEI SALEHI T., AMINI K., JAMSHIDIAN M.. SURVEY GENOTYPING OF ANIMAL AND HUMAN KLEBSIELLA PNEUMONIAE ISOLATES USING ERIC-PCR AND EVALUATION OF ANTIBIOTIC SENSITIVITY PATTERN. JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN[Internet]. 2018;15 (NEW)(2 (61) ):2469-2477. Available from: https://sid.ir/paper/714283/en

    IEEE: Copy

    E. ESTABRAGHI, T. ZAHRAEI SALEHI, K. AMINI, and M. JAMSHIDIAN, “SURVEY GENOTYPING OF ANIMAL AND HUMAN KLEBSIELLA PNEUMONIAE ISOLATES USING ERIC-PCR AND EVALUATION OF ANTIBIOTIC SENSITIVITY PATTERN,” JOURNAL OF COMPARATIVE PATHOBIOLOGY IRAN, vol. 15 (NEW), no. 2 (61) , pp. 2469–2477, 2018, [Online]. Available: https://sid.ir/paper/714283/en

    Related Journal Papers

  • No record.
  • Related Seminar Papers

  • No record.
  • Related Plans

  • No record.
  • Recommended Workshops

  • No record.





  • مرکز اطلاعات علمی SID
    strs
    دانشگاه امام حسین
    بنیاد ملی بازیهای رایانه ای
    کلید پژوه
    ایران سرچ
    ایران سرچ
    File Not Exists.
    Move to top