LIPASE SECRETION AND DOCOSAHEXAENOIC ACID PRODUCTION BY AURANTIOCHYTRIUM STRAIN GROWN ON FLAXSEED OIL

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AMOOZIAN N.; SHAKERI SH.; MALEKI M.; SHAKERI A.

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Journal: IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY Year:2017 | Volume:14 | Issue:70 Page(s): 123-134

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Title

LIPASE SECRETION AND DOCOSAHEXAENOIC ACID PRODUCTION BY AURANTIOCHYTRIUM STRAIN GROWN ON FLAXSEED OIL

Author(s)

AMOOZIAN N. | SHAKERI SH. | MALEKI M. | SHAKERI A. | Issue Writer Certificate

Keywords

AURANTIOCHYTRIUM STRAINQ1

OMEGA 3 OILQ1

DOCOSAHEXAENOIC ACIDQ2

FLAXSEED OILQ1

LINOLENIC ACIDQ1

Abstract

Omega-3 fatty acids are a large group of polyunsaturated fatty acids. DOCOSAHEXAENOIC ACID, the most important omega-3 fatty acid, has beneficial effects on human health. The aim of this study was to convert FLAXSEED OIL by native strain of Aurantiochytrium to omega-3 oil rich in DHA and LINOLENIC ACID.At first, lipase production and its activity was measured in broth medium. Production of biomass and oil in medium containing amounts of FLAXSEED OIL were determined. Also, saturated and unsaturated fatty acids were determined by gas chromatography. AURANTIOCHYTRIUM STRAIN produced precipitated halozone after 48 hours incubation in lipase production medium with a diameter of 6 mm. Lipase activity of 150 units per ml was measured in medium containing 1% of FLAXSEED OIL. atty acids oil in basal medium without FLAXSEED OIL was including C14, C15, C16, C17, C18, C18: 1, DPA and DHA. By adding FLAXSEED OIL to the basal medium, biomass was increased to 15.7 g/l. The greatest amount of DHA (388 mg/l) and oil content (53.25%) was obtained in medium containing 0.6% of FLAXSEED OIL. In conclusion, by increasing the concentration of FLAXSEED OIL in medium, the amount of saturated fatty acids was decreased and mono or polyunsaturated fatty acids were increased. Also, fatty acids profile of oil produced in FLAXSEED OIL medium was including C18: 2, C18: 3, DPA and DHA. Finally, produced omega-3 oil was contained DHA, linolenic and oleic acid and can be used to enrichment foods and dairy products.

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APA: Copy

AMOOZIAN, N., SHAKERI, SH., MALEKI, M., & SHAKERI, A.. (2017). LIPASE SECRETION AND DOCOSAHEXAENOIC ACID PRODUCTION BY AURANTIOCHYTRIUM STRAIN GROWN ON FLAXSEED OIL. IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 14(70), 123-134. SID. https://sid.ir/paper/71562/en

Vancouver: Copy

AMOOZIAN N., SHAKERI SH., MALEKI M., SHAKERI A.. LIPASE SECRETION AND DOCOSAHEXAENOIC ACID PRODUCTION BY AURANTIOCHYTRIUM STRAIN GROWN ON FLAXSEED OIL. IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY[Internet]. 2017;14(70):123-134. Available from: https://sid.ir/paper/71562/en

IEEE: Copy

N. AMOOZIAN, SH. SHAKERI, M. MALEKI, and A. SHAKERI, “LIPASE SECRETION AND DOCOSAHEXAENOIC ACID PRODUCTION BY AURANTIOCHYTRIUM STRAIN GROWN ON FLAXSEED OIL,” IRANIAN JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, vol. 14, no. 70, pp. 123–134, 2017, [Online]. Available: https://sid.ir/paper/71562/en

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