Identification of Phomopsis Species on Some Ornamental and Forest Plants in Iran on the Basis of the Morphological and Molecular Characteristics

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Bavand Savadkuhi Surena; Mahdian Safarali; BABAEIZAD VALIOLLAH; Tajik Ghanbari Mohammadali

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF ORNAMENTAL PLANTS (JOURNAL OF ORNAMENTAL AND HORTICULTURAL PLANTS) Year:2019 | Volume:9 | Issue:3 Page(s): 223-231

Download Full-Text

View:

218

Download:

269

Cites:

Information Journal Paper

Title

Identification of Phomopsis Species on Some Ornamental and Forest Plants in Iran on the Basis of the Morphological and Molecular Characteristics

Author(s)

Bavand Savadkuhi Surena | Mahdian Safarali | BABAEIZAD VALIOLLAH | Tajik Ghanbari Mohammadali | Issue Writer Certificate

Keywords

Molecular identification

Morphological identification

Phomopsis

Abstract

Phomopsis is a genus of imperfect plant pathogenic fungus whose hosts comprise several species in different regions of the world, such as grapes, soybean, acacia, hollyhock, velvetleaf, and several other plants. In this study, samples were collected from hollyhock, velvetleaf, purple bauhinia, and acacia plants suspected to be infected with Phomopsis fungi. They were, then, cultured and subjected to the separation and purification steps, and morphological characteristics of the derived isolates were determined. Among the isolates, five representatives (P1 to P5) were selected from Mazandaran province and their DNAs were extracted. The TEF and ITS regions of DNAs were amplified and sequenced. The ITS (4 and 5) region from ribosomal DNA and a part of the TEF gene were amplified and sequenced. The nucleotide sequence of the isolates was compared with the corresponding sequences available in the GeneBank, and the phylogenetic tree of the isolates was constructed. Results showed that the sequence of the isolates P1, P2, and P4 had the highest similarity with Phomopsis malvacearum species. The sequence of the isolate P3 was very similar to Phomopsis loropetali, and the sequence of the isolate P5 was very similar to Phomopsis theicola. The average size of pycnidia produced on three culture media, i. e. CDA, CMA, and PDA, was measured 16 days after incubation at 20-25° C. Sizes were 256 × 85 μ m in acacia, 230 × 68 μ m in velvetleaf, 230 × 69 μ m in hollyhock and 193 × 47 μ m in purple bauhinia isolates. Morphological and molecular features of the identified species were matched. The species identified here are reported from Iran for the first time. Symptoms of Phomopsis infection varied with host and identified species.

Multimedia

No record.

Cites

No record.

References

No record.

Cite

APA: Copy

Bavand Savadkuhi, Surena, Mahdian, Safarali, BABAEIZAD, VALIOLLAH, & Tajik Ghanbari, Mohammadali. (2019). Identification of Phomopsis Species on Some Ornamental and Forest Plants in Iran on the Basis of the Morphological and Molecular Characteristics. JOURNAL OF ORNAMENTAL PLANTS (JOURNAL OF ORNAMENTAL AND HORTICULTURAL PLANTS), 9(3 ), 223-231. SID. https://sid.ir/paper/741304/en

Vancouver: Copy

Bavand Savadkuhi Surena, Mahdian Safarali, BABAEIZAD VALIOLLAH, Tajik Ghanbari Mohammadali. Identification of Phomopsis Species on Some Ornamental and Forest Plants in Iran on the Basis of the Morphological and Molecular Characteristics. JOURNAL OF ORNAMENTAL PLANTS (JOURNAL OF ORNAMENTAL AND HORTICULTURAL PLANTS)[Internet]. 2019;9(3 ):223-231. Available from: https://sid.ir/paper/741304/en

IEEE: Copy

Surena Bavand Savadkuhi, Safarali Mahdian, VALIOLLAH BABAEIZAD, and Mohammadali Tajik Ghanbari, “Identification of Phomopsis Species on Some Ornamental and Forest Plants in Iran on the Basis of the Morphological and Molecular Characteristics,” JOURNAL OF ORNAMENTAL PLANTS (JOURNAL OF ORNAMENTAL AND HORTICULTURAL PLANTS), vol. 9, no. 3 , pp. 223–231, 2019, [Online]. Available: https://sid.ir/paper/741304/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops