A STUDY ON THE EFFECT OF CHEMICAL AND ENZYMATIC FACTORS ON THE SOLUBILITY OF SHRIMP HEAD WASTE PROTEINS

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MIZANI MARYAM; AMIN LARI M.; KHODABANDEH M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Year:2004 | Volume:10 | Issue:2 Page(s): 59-72

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Information Journal Paper

Title

A STUDY ON THE EFFECT OF CHEMICAL AND ENZYMATIC FACTORS ON THE SOLUBILITY OF SHRIMP HEAD WASTE PROTEINS

Author(s)

MIZANI MARYAM | AMIN LARI M. | KHODABANDEH M. | Issue Writer Certificate

Keywords

SHRIMP HEAD WASTEQ4

ALCALASEQ4

PROTEIN EXTRACTIONQ4

Abstract

SHRIMP HEAD WASTE (SHW) has been studied as a valuable source of protein (64.1%, dwb). In the present research the effect of different factors including pH, chemical agents such as NaCl, NaSO3, Triton x-100 and also application of commercial proteolytic enzyme, ALCALASE , on the solubility of SHW proteins and electrophoresis patterns of the extracted proteins was investigated. The results show that pH=8 is the optimum for PROTEIN EXTRACTION. Three of the factors (i.e. NaS03 Triton x-100 and ALCALASE) improve the results successfully, at their optimum concentration (200 mmol/l, 1% and 0.5%) respectively. Enzymatic treatment, using ALCALASE, alone or in combination with NaSO3 for Triton x-100 resulted in higher solubilization of proteins. SDS-P AGE patterns revealed appearance of small molecular weight proteins, less than 14-20 KDa, due to chemical or enzymatic treatments, which probably contribute to the higher solubility. These results indicate that it is possible to improve protein solubility by treatment with NaSO3, Alca-lase or in combination of both.

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APA: Copy

MIZANI, MARYAM, AMIN LARI, M., & KHODABANDEH, M.. (2004). A STUDY ON THE EFFECT OF CHEMICAL AND ENZYMATIC FACTORS ON THE SOLUBILITY OF SHRIMP HEAD WASTE PROTEINS. JOURNAL OF AGRICULTURAL SCIENCES, 10(2), 59-72. SID. https://sid.ir/paper/7624/en

Vancouver: Copy

MIZANI MARYAM, AMIN LARI M., KHODABANDEH M.. A STUDY ON THE EFFECT OF CHEMICAL AND ENZYMATIC FACTORS ON THE SOLUBILITY OF SHRIMP HEAD WASTE PROTEINS. JOURNAL OF AGRICULTURAL SCIENCES[Internet]. 2004;10(2):59-72. Available from: https://sid.ir/paper/7624/en

IEEE: Copy

MARYAM MIZANI, M. AMIN LARI, and M. KHODABANDEH, “A STUDY ON THE EFFECT OF CHEMICAL AND ENZYMATIC FACTORS ON THE SOLUBILITY OF SHRIMP HEAD WASTE PROTEINS,” JOURNAL OF AGRICULTURAL SCIENCES, vol. 10, no. 2, pp. 59–72, 2004, [Online]. Available: https://sid.ir/paper/7624/en

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