CELL SUSPENSION AND PROTOPLAST CULTURE IN RICE (ORYZA SATIVA L.) CULTIVARS

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KHALILI MOUSAVI AZAD; GHORBANLI MAH LAGHA; EBRAHIMZADEH H.; NEMATZADEH GH.

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Journal: Year:2002 | Volume:8 | Issue:3 Page(s): 43-61

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Title

CELL SUSPENSION AND PROTOPLAST CULTURE IN RICE (ORYZA SATIVA L.) CULTIVARS

Author(s)

KHALILI MOUSAVI AZAD | GHORBANLI MAH LAGHA | EBRAHIMZADEH H. | NEMATZADEH GH. | Issue Writer Certificate

Keywords

RICEQ2

CELL SUSPENSIONQ4

CALLUSQ2

Abstract

This study aim at: investigating CELL SUSPENSION culture, initiation and maintaining estabishing an embryogenic suspension, ability regeneration from suspension culture, isolation and protoplast culture in many RICE cultivars: Hasan saraii, Hasani, Domsiah, Nemat, Neda and Khazar.Embryogenic CALLUS initiated from mature seed RICE cultivars (Ms medium containing various concentration of 2,4-D and BAP (0/2 mg.L)) was used to establish embryogenic CELL SUSPENSION Cultures, CELL SUSPENSION was established in R2 liquid culture medium containing 2,4-D (2mg/L) in dark.The result shows CALLUS of the Hasan saraii and domsiah cultivars proliferated fasty and estahlished CELL SUSPENSION formed in the suhcultures. Transfered CALLUS in R2 liquid without 2,4-D was formed somatic embryo (gloubular and heart - from stages), CALLUS were maintained the ability to proliferat and differentiate for long periods of time (2 years). The process of embryogenesis can he divided into four different stages: initiation or induction, proliferation, development or maturation, and finally germination. During initiation, cells are indued to form somatic embryos; during proliferation, these induced cells undergi multiplication with very little or no maturation of the embryogenic tissue, germination is inclued in the process of somatic embryogenesis.Callus Hasani, Nemat and Neda cultivar to bacome brown and dead after almost one month of transfer in R2 liquid culture medium.The CALLUS Kazar cultivar maintained the ability to proliferat during one month but no proliforating in second month in R2 liquid medium was observed and it didnot show somatic embryo, and showed only cells aggregate with gloubular cells at the outer surface.Regeneration frequency of CELL SUSPENSION observed very little. Three and one plantlets regenerated from Domsiah and Hasn sarii, respectively, in semi medium (LS containing BAP (1mg/L), NAA (0/5mg/L)).Protoplasts isolated from 6, 8, 10, 12, 15 month CELL SUSPENSION Hasan saraii and Domsiah (Enzyme soulation containing sellulase, pectolyse, macerozyme).Before one month of protoplasts culture from Domsiah cultivar formed microcallus was formed, hut didnot showed late growth.

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APA: Copy

KHALILI MOUSAVI, AZAD, GHORBANLI, MAH LAGHA, EBRAHIMZADEH, H., & NEMATZADEH, GH.. (2002). CELL SUSPENSION AND PROTOPLAST CULTURE IN RICE (ORYZA SATIVA L.) CULTIVARS. JOURNAL OF AGRICULTURAL SCIENCES, 8(3), 43-61. SID. https://sid.ir/paper/7654/en

Vancouver: Copy

KHALILI MOUSAVI AZAD, GHORBANLI MAH LAGHA, EBRAHIMZADEH H., NEMATZADEH GH.. CELL SUSPENSION AND PROTOPLAST CULTURE IN RICE (ORYZA SATIVA L.) CULTIVARS. JOURNAL OF AGRICULTURAL SCIENCES[Internet]. 2002;8(3):43-61. Available from: https://sid.ir/paper/7654/en

IEEE: Copy

AZAD KHALILI MOUSAVI, MAH LAGHA GHORBANLI, H. EBRAHIMZADEH, and GH. NEMATZADEH, “CELL SUSPENSION AND PROTOPLAST CULTURE IN RICE (ORYZA SATIVA L.) CULTIVARS,” JOURNAL OF AGRICULTURAL SCIENCES, vol. 8, no. 3, pp. 43–61, 2002, [Online]. Available: https://sid.ir/paper/7654/en

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