Analyzing Simple Sequence Repeats Derived from Expressed Sequence Tags in Dromedary Camels

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BARAZANDEH A.; MOKHTARI M.; MOGHBELI DAMANEH M.; ROUDBARI Z.

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Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE Year:2020 | Volume:10 | Issue:3 Page(s): 555-565

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Title

Analyzing Simple Sequence Repeats Derived from Expressed Sequence Tags in Dromedary Camels

Author(s)

BARAZANDEH A. | MOKHTARI M. | MOGHBELI DAMANEH M. | ROUDBARI Z. | Issue Writer Certificate

Keywords

camel

EST

gene ontology

genomic

molecular marker

SSR

Abstract

The objective of the current research was to make a character description of simple sequence repeats (SSR) derived from expressed sequence TAGs (EST) markers of dromedary camels (EST-SSR markers) and to conduct a practical analysis of these sequences for their application in comparative genomics and molecular genetics studies. A complete of 862 SSRs were discovered from 17155 EST sequences using the SSR Loca-tor software. 827 EST out of 17155 EST had SSRs, that 794 (96%), 31 (3. 8%) and 2 (0. 2%) of them con-tained 1, 2 and 3 SSRs, respectively. The dimeric motifs were the most abundant SSRs (38. 86%), followed by 27. 15%, 21. 46%, 6. 96%, and 5. 57% for tri-, hexa-, tetra-and pentameric motifs. The most plentiful dimer, trimer, tetramer, pentamer and hexamer motif were AC/TG (54%), GCC/GGC (19. 2%), TTTA (13. 3%), AAAAG (10. 4%) and AACCAC (67. 6 %), respectively. BLASTX was used to examine the final non-redundant EST-SSRs. Almost all of EST-SSRs were found out to be protected in the macromolecule catabolic process and RNA processing and splicing. EST-SSR markers might be applied as a novel resource of useful markers in the biological survey. Also, these markers may be a valuable source for further molecular genetics and genomics research of camels and related species.

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APA: Copy

BARAZANDEH, A., MOKHTARI, M., MOGHBELI DAMANEH, M., & ROUDBARI, Z.. (2020). Analyzing Simple Sequence Repeats Derived from Expressed Sequence Tags in Dromedary Camels. IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE, 10(3), 555-565. SID. https://sid.ir/paper/782486/en

Vancouver: Copy

BARAZANDEH A., MOKHTARI M., MOGHBELI DAMANEH M., ROUDBARI Z.. Analyzing Simple Sequence Repeats Derived from Expressed Sequence Tags in Dromedary Camels. IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE[Internet]. 2020;10(3):555-565. Available from: https://sid.ir/paper/782486/en

IEEE: Copy

A. BARAZANDEH, M. MOKHTARI, M. MOGHBELI DAMANEH, and Z. ROUDBARI, “Analyzing Simple Sequence Repeats Derived from Expressed Sequence Tags in Dromedary Camels,” IRANIAN JOURNAL OF APPLIED ANIMAL SCIENCE, vol. 10, no. 3, pp. 555–565, 2020, [Online]. Available: https://sid.ir/paper/782486/en

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