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Information Journal Paper

Title

FACTORS AFFECTING MICROSHOOT ROOTING OF PERSIAN WALNUT (JUGLANS REGIA L.)

Pages

  135-146

Abstract

 Rooting is the main limiting factor in the MICROPROPAGATION of PERSIAN WALNUT (Juglans regia L.) due to the poor ROOTING capacity of this species. Two experiments were conducted to identify a suitable procedure for ROOTING of walnut microshoots. ROOTING was performed in two successive phases: A) root induction and B), root development. In the first experiment, roots were induced in five different compositions of the basal nutrient media in darkness. After three weeks, for root development, the basal ends of shoots were dipped in talcum powder containing 2% IBA and were transferred to moisted Jiffy pots. Maximum ROOTING (42.5%) was obtaind by microshoots cultured on DKW medium (1/2-strength macronutrients) with full calcium nitrate which was signuficantly greater than other treatments. In another experiment, for root induction shoots were cultured on DKW medium (1/2-strength macronutrients) containing 0.2 mg 1-1 NAA and 3.0 g mg 1-1 activated charcoal for three weeks under lighted conditions. Then, the basal ends of shoots were dipped in 6 different ROOTING powders or auxin solutions containing different concentrations of IBA and were transferred to Jiffy pots. Maximum ROOTING (29.17%) was obtained by basal dip immersion of shoots in a commercial ROOTING powder (Seradix 3), containing 0.8% IBA. The use of Jiffy pots allowed rapid development of the root system and improvement in the quality of plantlets (higher number of roots, presence of secondary roots) and root development was synchronized with shoot elongation and development of new leaves. On transferring the plantlets to the greenhouse, their growth continued and more than 90% of plantlets survived.

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    APA: Copy

    SAADAT, Y.A., & HENNERTY, M.J.. (2000). FACTORS AFFECTING MICROSHOOT ROOTING OF PERSIAN WALNUT (JUGLANS REGIA L.). IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, 1(3-4), 135-146. SID. https://sid.ir/paper/80868/en

    Vancouver: Copy

    SAADAT Y.A., HENNERTY M.J.. FACTORS AFFECTING MICROSHOOT ROOTING OF PERSIAN WALNUT (JUGLANS REGIA L.). IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY[Internet]. 2000;1(3-4):135-146. Available from: https://sid.ir/paper/80868/en

    IEEE: Copy

    Y.A. SAADAT, and M.J. HENNERTY, “FACTORS AFFECTING MICROSHOOT ROOTING OF PERSIAN WALNUT (JUGLANS REGIA L.),” IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, vol. 1, no. 3-4, pp. 135–146, 2000, [Online]. Available: https://sid.ir/paper/80868/en

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