A COMPARISON OF DIFFERENT POPULATIONS OF TEA ROOT LESION NEMATODE, PRATYLENCHUS LOOS; LOOF, 1960, FROM GUILAN PROVINCE, IRAN'S TEA GARDENS BASED ON ITS RDNA AND D2/D3 LSU-RDNA GENOMIC REGIONS PCR- RFLPS

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HAJI EGHRARI B.; MOHAMMADI MOJTABA; KHEYRI A.; TANHA MOAFI Z.

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Paper Information

Journal: IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE) Year:2007 | Volume:37 | Issue:6 Page(s): 965-971

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Title

A COMPARISON OF DIFFERENT POPULATIONS OF TEA ROOT LESION NEMATODE, PRATYLENCHUS LOOS; LOOF, 1960, FROM GUILAN PROVINCE, IRAN'S TEA GARDENS BASED ON ITS RDNA AND D2/D3 LSU-RDNA GENOMIC REGIONS PCR- RFLPS

Author(s)

HAJI EGHRARI B. | MOHAMMADI MOJTABA | KHEYRI A. | TANHA MOAFI Z. | Issue Writer Certificate

Keywords

TEAQ3

PRATYLENCHLLS LOOSIQ3

PCR-RFLPQ3

I TS-RDNAQ3

D2/D3 LSU-RDNAQ3

Abstract

In order to study the genomic variation of TEA root lesion nematode, Pratylellchs loosi Loof, 1960, from infested TEA gardens IS samples were collected from different regions of Guilan province. The samples (in plastic bags) were transferred into the nematology lab and stored in suitable needed humidity and 5-10.C in refrigerating conditions before being processed. After extraction of the nematode, template DNAs were extracted from each population, and ITS-rDNA as well as D2/D3 LSU - rDNA genomic regions were amplified by polymer asechain reaction (PCR) while using special primers. Each amplified genomic region was digested by endonuclease restricting enzymes including DraI, PstI, HindllI, HindU, MboI, Hin61, AluI, and HaelII. Results indicated that the amplification of ITS- rDNA and D2/D3 LSU. rDNA genomic regions by PCR, introduced unit genomic bands (respectively 1250 and 787 pb. in size) about each 0 I them, and in all studied populations. These results also showed that there are much similarities among produced genomic segments through endonuclease restricting enzymes. ITS-rDNA genomic region was not restricted by HaeIII and D2/D3 LSU - rDNA, neigher was by PstI; DraI and HindIII .

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References

جداسازی نماتد Pratylenchus loosi از روی نهال های وارداتی چای از ژاپن

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APA: Copy

HAJI EGHRARI, B., MOHAMMADI, MOJTABA, KHEYRI, A., & TANHA MOAFI, Z.. (2007). A COMPARISON OF DIFFERENT POPULATIONS OF TEA ROOT LESION NEMATODE, PRATYLENCHUS LOOS; LOOF, 1960, FROM GUILAN PROVINCE, IRAN'S TEA GARDENS BASED ON ITS RDNA AND D2/D3 LSU-RDNA GENOMIC REGIONS PCR- RFLPS. IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE), 37(6), 965-971. SID. https://sid.ir/paper/8395/en

Vancouver: Copy

HAJI EGHRARI B., MOHAMMADI MOJTABA, KHEYRI A., TANHA MOAFI Z.. A COMPARISON OF DIFFERENT POPULATIONS OF TEA ROOT LESION NEMATODE, PRATYLENCHUS LOOS; LOOF, 1960, FROM GUILAN PROVINCE, IRAN'S TEA GARDENS BASED ON ITS RDNA AND D2/D3 LSU-RDNA GENOMIC REGIONS PCR- RFLPS. IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE)[Internet]. 2007;37(6):965-971. Available from: https://sid.ir/paper/8395/en

IEEE: Copy

B. HAJI EGHRARI, MOJTABA MOHAMMADI, A. KHEYRI, and Z. TANHA MOAFI, “A COMPARISON OF DIFFERENT POPULATIONS OF TEA ROOT LESION NEMATODE, PRATYLENCHUS LOOS; LOOF, 1960, FROM GUILAN PROVINCE, IRAN'S TEA GARDENS BASED ON ITS RDNA AND D2/D3 LSU-RDNA GENOMIC REGIONS PCR- RFLPS,” IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE), vol. 37, no. 6, pp. 965–971, 2007, [Online]. Available: https://sid.ir/paper/8395/en

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