DETECTION OF POTATO VIRUS Y BY REVERSE TRANSCRIPTION POLYMERASE CHANE REACTION (RT-PCR)

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GHOLAMI SARA; KOUHI HABIBI M.; BOUSHEHRI A.A.; NAGHAVI M.R.

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Journal: IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE) Year:2007 | Volume:38-1 | Issue:2 (AGRONOMY AND CROP BIOTECHNOLOGY) Page(s): 399-405

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Title

DETECTION OF POTATO VIRUS Y BY REVERSE TRANSCRIPTION POLYMERASE CHANE REACTION (RT-PCR)

Author(s)

GHOLAMI SARA | KOUHI HABIBI M. | BOUSHEHRI A.A. | NAGHAVI M.R. | Issue Writer Certificate

Keywords

POTATO VIRUS YQ4

POTYVIRUSQ2

PROTEIN COATINGQ3

RT-PCRQ2

Abstract

POTATO VIRUS Y (PVY) a member of the largest family of viruses, Potyviridae, is capable of infecting a large range of plants in several families causing an economically devastating disease. In order to manage viral diseases, sensitive and accurate detection methods are required. In this study, RT-PCR was employed to detect POTATO VIRUS Y in samples collected from fields in Tehran province. DAS-ELISA test was initially carried out for all samples and the infected samples were inoculated to test plants (using phosphate buffer). Following total RNA extraction, with a pair of primers complimentary to the 5' non- translated terminal and pi protein region, RT-PCR as well as immunocapture RT-PCR were carried out. A 974bp fragment was amplified enabling us to recognize all known strains with a high sensitivity. It was further shown that, the plants which were kept at -20°C for up to 5 months did not show any significant differences in PCR reaction.

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APA: Copy

GHOLAMI, SARA, KOUHI HABIBI, M., BOUSHEHRI, A.A., & NAGHAVI, M.R.. (2007). DETECTION OF POTATO VIRUS Y BY REVERSE TRANSCRIPTION POLYMERASE CHANE REACTION (RT-PCR). IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE), 38-1(2 (AGRONOMY AND CROP BIOTECHNOLOGY)), 399-405. SID. https://sid.ir/paper/8448/en

Vancouver: Copy

GHOLAMI SARA, KOUHI HABIBI M., BOUSHEHRI A.A., NAGHAVI M.R.. DETECTION OF POTATO VIRUS Y BY REVERSE TRANSCRIPTION POLYMERASE CHANE REACTION (RT-PCR). IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE)[Internet]. 2007;38-1(2 (AGRONOMY AND CROP BIOTECHNOLOGY)):399-405. Available from: https://sid.ir/paper/8448/en

IEEE: Copy

SARA GHOLAMI, M. KOUHI HABIBI, A.A. BOUSHEHRI, and M.R. NAGHAVI, “DETECTION OF POTATO VIRUS Y BY REVERSE TRANSCRIPTION POLYMERASE CHANE REACTION (RT-PCR),” IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE), vol. 38-1, no. 2 (AGRONOMY AND CROP BIOTECHNOLOGY), pp. 399–405, 2007, [Online]. Available: https://sid.ir/paper/8448/en

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