PURIFICATION, ANTISERUM PREPARATION AND SEROLOGICAL IDENTIFICATION OF POTATO Y AND X VIRUSES ISOLATED FROM POTATO FIELDS OF KHUZESTAN PROVINCE

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SHAMSBAKHSH M.; KHAKVAR R.; POURRAHIM R.

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Paper Information

Journal: THE SCIENTIFIC JOURNAL OF AGRICULTURE (SJA) Year:2001 | Volume:24 | Issue:1 Page(s): 35-47

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Title

PURIFICATION, ANTISERUM PREPARATION AND SEROLOGICAL IDENTIFICATION OF POTATO Y AND X VIRUSES ISOLATED FROM POTATO FIELDS OF KHUZESTAN PROVINCE

Author(s)

SHAMSBAKHSH M. | KHAKVAR R. | POURRAHIM R. | Issue Writer Certificate

Keywords

POTATO Y VIRUSQ4

POTATO X VIRUSQ3

ELISAAND DIBAQ3

Abstract

The most important viruses of potato are potato viruses Y and X. The Purpose of the present study was to purify PVY and PVX isolated from potato fields of Khuzestan province and to prepare an antiserum against each of them in order to develop a sensitive serological test for diagnosis of these viruses. Potato Y and X viruses were isolated from potato fields in Khuzestan and propagated in Nicotiana tabacum cv. Samsum under greenhose conditions. Both viruses were purified by a modification of the method of McDonald et al., 1976. Rabbits were used to raise polyclonal antisera against each of the purified viruses. The produced antisera had a titer of 128 for PVY and 256 for PVX in microprecipitin test. Enzyme linked immunosorbent assay (ELISA) and dot immuno-binding assay (DIBA) systems were developed for both viruses, were quite suitable for detecting viruses in infected plants. No reaction was obtained between the antisera and healthy potato sap.

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APA: Copy

SHAMSBAKHSH, M., KHAKVAR, R., & POURRAHIM, R.. (2001). PURIFICATION, ANTISERUM PREPARATION AND SEROLOGICAL IDENTIFICATION OF POTATO Y AND X VIRUSES ISOLATED FROM POTATO FIELDS OF KHUZESTAN PROVINCE. THE SCIENTIFIC JOURNAL OF AGRICULTURE (SJA), 24(1), 35-47. SID. https://sid.ir/paper/24725/en

Vancouver: Copy

SHAMSBAKHSH M., KHAKVAR R., POURRAHIM R.. PURIFICATION, ANTISERUM PREPARATION AND SEROLOGICAL IDENTIFICATION OF POTATO Y AND X VIRUSES ISOLATED FROM POTATO FIELDS OF KHUZESTAN PROVINCE. THE SCIENTIFIC JOURNAL OF AGRICULTURE (SJA)[Internet]. 2001;24(1):35-47. Available from: https://sid.ir/paper/24725/en

IEEE: Copy

M. SHAMSBAKHSH, R. KHAKVAR, and R. POURRAHIM, “PURIFICATION, ANTISERUM PREPARATION AND SEROLOGICAL IDENTIFICATION OF POTATO Y AND X VIRUSES ISOLATED FROM POTATO FIELDS OF KHUZESTAN PROVINCE,” THE SCIENTIFIC JOURNAL OF AGRICULTURE (SJA), vol. 24, no. 1, pp. 35–47, 2001, [Online]. Available: https://sid.ir/paper/24725/en

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