PREPARATION AND EVALUATION OF AN INACTIVATED H9N2 AVIAN INFLUENZA ANTIGEN FOR USING IN HAEMAGGLUTINATION INHIBITION TEST

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VASFI MARANDI M.; BOZORG MEHRIFARD M.H.

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Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR) Year:2001 | Volume:2 | Issue:2 Page(s): 174-181

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Title

PREPARATION AND EVALUATION OF AN INACTIVATED H9N2 AVIAN INFLUENZA ANTIGEN FOR USING IN HAEMAGGLUTINATION INHIBITION TEST

Author(s)

VASFI MARANDI M. | BOZORG MEHRIFARD M.H. | Issue Writer Certificate

Keywords

AVIAN INFLUENZAQ4

INACTIVAED ANTIGENQ4

HAEMAGGLUTINATIONQ3

HAEMAGGLUTINATION INHIBITIONQ4

Abstract

Serological screening of chicken’s sera for the presence of H9N2 subtype of AVIAN INFLUENZA virus (AIV) specific antibodies in Iran is currently carried out by HAEMAGGLUTINATION inhibition (ID) test in avian diagnostic laboratories. Therefore, there is a need to prepare an inactivated H9N2 antigen for routine and large scale epidemiological surveillance and control of H9N2 outbreaks. Three non-pathogenic H9N2 AIV strains, isolated during the initial AVIAN INFLUENZA (AI) outbreak, were primerely selected to compare their growth in embryonated chicken eggs. A H9N2 AIV isolate designatedas A/chicken/Iran/ZMT-101/98 was provided high yeiled amnioallantoic fluid (AAF) with an average of 10.5 HAEMAGGLUTINATION(HA), 109.9 EL050 titers and 3.2 days (77h) MOT, as compared with two other homologous strains. The results obtained indicated that 0.1 percent formalin achieve complete virus inactivation with low destroying effect on HA and HI titers. This antigen was able to detect specific antibody as early as 6 days after post inoculation in HI test, indicating that HI is a reliable, specific and rapid srological test. Comparison of HI and ELISA tests to detect H9N2 infection in the sera obtained during 2 and 4 wks after acute phase of disease showed respectively 94.44 and 98.61 percent similarity. Overall, it is suggested that inactivated H9N2 antigen could successfully be use by avian diagnostic laboratories for routine flocks monitoring and regulatory surveillance of poultry industry.

Cites

A SEROEPIDEMIOLOGIC STUDY OF AVIAN INFLUENZA (H9N2) IN IRAN

References

SEROLOGICAL MONITORING OF HAEMAGGLUTINATION INHIBITION ANTIBODIES IN A MULTI AGED LAYER FARM FOLLOWING AVIAN INFLUENZA OUTBREAK DUE TO H9N2 SUB-SEROTYPE IN 1998 IN IRAN

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APA: Copy

VASFI MARANDI, M., & BOZORG MEHRIFARD, M.H.. (2001). PREPARATION AND EVALUATION OF AN INACTIVATED H9N2 AVIAN INFLUENZA ANTIGEN FOR USING IN HAEMAGGLUTINATION INHIBITION TEST. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), 2(2), 174-181. SID. https://sid.ir/paper/85782/en

Vancouver: Copy

VASFI MARANDI M., BOZORG MEHRIFARD M.H.. PREPARATION AND EVALUATION OF AN INACTIVATED H9N2 AVIAN INFLUENZA ANTIGEN FOR USING IN HAEMAGGLUTINATION INHIBITION TEST. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR)[Internet]. 2001;2(2):174-181. Available from: https://sid.ir/paper/85782/en

IEEE: Copy

M. VASFI MARANDI, and M.H. BOZORG MEHRIFARD, “PREPARATION AND EVALUATION OF AN INACTIVATED H9N2 AVIAN INFLUENZA ANTIGEN FOR USING IN HAEMAGGLUTINATION INHIBITION TEST,” IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), vol. 2, no. 2, pp. 174–181, 2001, [Online]. Available: https://sid.ir/paper/85782/en

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