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Information Journal Paper

Title

POLYMORPHISM OF SHEEPPOU1F1 GENE EXON 6 AND 3' UTR REGION AND THEIR ASSOCIATION WITH MILK PRODUCTION TRAITS

Pages

  331-335

Abstract

 The purpose of the study was to detect the Alu I and Dde I POLYMORPHISMs within POU1F1 GENE exon 6 and 3' UTR region in TURKISH SHEEP BREEDS, and their association with milk productive traits. Jugular blood samples were collected from 50 Sakiz, 50 White Karaman, and 50 Awassi sheep using EDTA as an anticoagulant. PCR-RFLP and sequencing analysis were performed to investigate possible POLYMORPHISMs in the exon 6 and 3' flanking region of the sheepPOU1F1 gene. The PCR products were digested with restriction endonucleaseAlu I and Dde I, and biallelic POLYMORPHISM was found with restriction endonuclease Alu I and two genotypes (TT (296 bp and 173 bp) and CC (235 bp, 173 bp and 61 bp)) were detected. White Karaman and Awassi breeds did not show POLYMORPHISMs forAlu I restriction sites. No POLYMORPHISM at the Dde I cleavage sites was detected in the three sheep breeds. Significant statistical results were found in milk yield (***P<0.001), fat (***P<0.001) and lactose (*P<0.05) values with TT and CCgenotypes, however no significant association of TT and CC genotypes with protein values was detected (P>0.05) and individuals with genotypeTT had a superior milk yield in Sakiz sheep breeds. As sequence results, seven variation points were determined for exon 6 (g.185T>C) and 3' UTR (g.220G>A, g.229C>T, g.248C>T, g.250A>T, g.255T>C, g.258C>T) of the sheepPOU1F1 gene. We have reported here for the first time single nucleotide POLYMORPHISMs of thePOU1F1 gene for both exon 6 and 3' UTR and its effects on MILK TRAITS in TURKISH SHEEP BREEDS were evaluated.

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    APA: Copy

    OZMEN, O., KUL, S., & UNAL, E.O.. (1393). پلی مورفیسم اکسون 6 ژن pou1f1 و ناحیه 3’utr گوسفندی و رابطه آن ها با صفات تولید شیر. مجله تحقیقات دامپزشکی ایران (دانشگاه شیراز), 15(4 (مسلسل 49) ), 331-335. SID. https://sid.ir/paper/85819/en

    Vancouver: Copy

    OZMEN O., KUL S., UNAL E.O.. پلی مورفیسم اکسون 6 ژن pou1f1 و ناحیه 3’utr گوسفندی و رابطه آن ها با صفات تولید شیر. مجله تحقیقات دامپزشکی ایران (دانشگاه شیراز)[Internet]. 1393;15(4 (مسلسل 49) ):331-335. Available from: https://sid.ir/paper/85819/en

    IEEE: Copy

    O. OZMEN, S. KUL, and E.O. UNAL, “پلی مورفیسم اکسون 6 ژن pou1f1 و ناحیه 3’utr گوسفندی و رابطه آن ها با صفات تولید شیر,” مجله تحقیقات دامپزشکی ایران (دانشگاه شیراز), vol. 15, no. 4 (مسلسل 49) , pp. 331–335, 1393, [Online]. Available: https://sid.ir/paper/85819/en

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