EFFECT OF CULTURE MEDIA, EXPLANTS AND GROWTH REGULATORS ON CALLUS INDUCTION AND TAXOL PRODUCTION IN TAXUS BACCATA L.

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

YARI KHOSROUSHAHI A.; VALIZADEH M.; GHASEMPOUR A.R.; KHOSROU SHAHLI M.; NAGHDIBADI H.A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE) Year:2006 | Volume:37-1 | Issue:1 (AGRONOMY & CROP BIOTECHNOLOGY) Page(s): 69-76

Download Full-Text

Persian Verion

View:

1,046

Download:

Cites:

Information Journal Paper

Title

EFFECT OF CULTURE MEDIA, EXPLANTS AND GROWTH REGULATORS ON CALLUS INDUCTION AND TAXOL PRODUCTION IN TAXUS BACCATA L.

Author(s)

Keywords

TAXUS BACCATA L.Q3

TAXOLQ3

TISSUE CULTUREQ4

GROWTH REGULATORSQ3

CALLUS INDUCTIONQ3

Abstract

Taxol is a diterpenoid that is produced by Taxus Spp. (Yew trees). It is known as the most effective drug in cancer therapy. In order to improve CALLUS INDUCTION and TAXOL production in Taxus baccata some experiments were performed. These experiments were carried out using factorial design based on CRD. In all experiments, explants (stem and leaf), culture media (B5 and MS) and light conditions (dark and light) were studied. In addition, in the first experiment, BAP and Kin with constant levels of auxin were studied at two levels. In the second experiment, different ratios of auxines; NAA, 2, 4-D and cytokinin (Kin) were tested. Results indicated that the most CALLUS INDUCTION (97.46%) as well as callus growth (10 mm/two week) were obtained from stem explants on B5 medium supplemented by NAA (2 mg/l), 2,4-D( 0.2 mg/l) and Kin (0.2 mg/l). The light factor did not show any significant effect on either CALLUS INDUCTION or growth. In the third experiment, activated charcoal, polyvinyl pyrolidine (PVP), citric acid and ascorbic acid were added to the selected medium for inhibition of callus subculture browning. Results indicated that the above mentioned compounds well inhibited callus browning, but only the media containing ascorbic acid (50 mg/l) rendered suitable cell growth too. A comparison of xtracted TAXOL from light and brown calli through HPLC showed that TAXOL in light calli was 2.45 mg/kg, while being negligible in brown calli (lack of ascorbic acid).

Cites

THE EFFECT OF DIFFERENT CONCENTRATIONS 2,4-D ON CALLOGENESIS RATE OF TRIGONELLA FOENUM-GRAECUM L. INVITRO

References

EFFECT OF RELATIVE ILLUMINATION ON ESTABLISHING AND GROWTH OF COMMON YEW GRAFTS AND SAPLING

Cite

APA: Copy

YARI KHOSROUSHAHI, A., VALIZADEH, M., GHASEMPOUR, A.R., KHOSROU SHAHLI, M., & NAGHDIBADI, H.A.. (2006). EFFECT OF CULTURE MEDIA, EXPLANTS AND GROWTH REGULATORS ON CALLUS INDUCTION AND TAXOL PRODUCTION IN TAXUS BACCATA L.. IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE), 37-1(1 (AGRONOMY & CROP BIOTECHNOLOGY)), 69-76. SID. https://sid.ir/paper/8587/en

Vancouver: Copy

YARI KHOSROUSHAHI A., VALIZADEH M., GHASEMPOUR A.R., KHOSROU SHAHLI M., NAGHDIBADI H.A.. EFFECT OF CULTURE MEDIA, EXPLANTS AND GROWTH REGULATORS ON CALLUS INDUCTION AND TAXOL PRODUCTION IN TAXUS BACCATA L.. IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE)[Internet]. 2006;37-1(1 (AGRONOMY & CROP BIOTECHNOLOGY)):69-76. Available from: https://sid.ir/paper/8587/en

IEEE: Copy

A. YARI KHOSROUSHAHI, M. VALIZADEH, A.R. GHASEMPOUR, M. KHOSROU SHAHLI, and H.A. NAGHDIBADI, “EFFECT OF CULTURE MEDIA, EXPLANTS AND GROWTH REGULATORS ON CALLUS INDUCTION AND TAXOL PRODUCTION IN TAXUS BACCATA L.,” IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE), vol. 37-1, no. 1 (AGRONOMY & CROP BIOTECHNOLOGY), pp. 69–76, 2006, [Online]. Available: https://sid.ir/paper/8587/en

Related Journal Papers