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Information Journal Paper

Title

17α-HYDROXY-4-PREGENEN-3-ONE (17ΑP) ASSAY, USING ACETYLCHOLINESTERASE ENZYME AS TRACER

Pages

  13-28

Abstract

 17a-hydroxy-4-pregnen-3-one (17aP) hormone is a precursor of other steroid hormones and radioimmunoassay has already been used to measure it, but a simple and rapid "Enzyme Linked Immunosorbant Assay" (ELISA) is described and validated here. A general procedure for prepara-tion of the acetylcholinesterase labelled steroid is described, which is applicable to any steroid. Use of acetylcholinesterase tracer increased the sensitivity of assay so that reliable measurements of each steroid could be achieved with only 10m l of plasma. The ELISA was applied to measure 17, 20a-hydroxy-4-pregnen-3-one (17, 20aP) steroid production from 17aP by 20 a-hydroxy steroid dehydrogenase (20a-HSD) from sperm of ROACh (Rutilus rutilus). The results showed that cyprinid sperm contains potent and active 20a-HSD enzymes which produce 17, 20aP hormone from 17aP substrate.

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    APA: Copy

    EBRAHIMI, M.. (2005). 17α-HYDROXY-4-PREGENEN-3-ONE (17ΑP) ASSAY, USING ACETYLCHOLINESTERASE ENZYME AS TRACER . IRANIAN JOURNAL OF FISHERIES SCIENCES, 5(1), 13-28. SID. https://sid.ir/paper/85966/en

    Vancouver: Copy

    EBRAHIMI M.. 17α-HYDROXY-4-PREGENEN-3-ONE (17ΑP) ASSAY, USING ACETYLCHOLINESTERASE ENZYME AS TRACER . IRANIAN JOURNAL OF FISHERIES SCIENCES[Internet]. 2005;5(1):13-28. Available from: https://sid.ir/paper/85966/en

    IEEE: Copy

    M. EBRAHIMI, “17α-HYDROXY-4-PREGENEN-3-ONE (17ΑP) ASSAY, USING ACETYLCHOLINESTERASE ENZYME AS TRACER ,” IRANIAN JOURNAL OF FISHERIES SCIENCES, vol. 5, no. 1, pp. 13–28, 2005, [Online]. Available: https://sid.ir/paper/85966/en

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    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
    مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
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