A STUDY OF GENE TRANSFORMATION BY VACUUM INFILTRATION INTO RAPESEED AND ARABIDOPSIS PLANTS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

GHORBANI MESHKIN M.; JALALI JAVARAN MOKHTAR; SEPAHVAND N.A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE) Year:2006 | Volume:37-1 | Issue:3 (AGRONOMY & CROP BIOTECHNOLOGY) Page(s): 539-544

Download Full-Text

Persian Verion

View:

1,686

Download:

Cites:

Information Journal Paper

Title

A STUDY OF GENE TRANSFORMATION BY VACUUM INFILTRATION INTO RAPESEED AND ARABIDOPSIS PLANTS

Author(s)

GHORBANI MESHKIN M. | JALALI JAVARAN MOKHTAR | SEPAHVAND N.A. | Issue Writer Certificate

Keywords

GENE TRANSFORMATIONQ2

ARABIDOPSISQ3

BRASSICA NAPUSQ2

VACUUM INFILTRATIONQ3

GDS GENEQ4

Abstract

Different gene transfer strategies, based on tissue culture, are employed in plants such as rapeseed. These strategies suffer from constraints like; need of transgenic cell regeneration, somaclonal variation, in ad9ition to being costly and time consuming. Considering the constraints, researchers often resort to planta transformation. This strategy, in addition to simplicity, low cost, and avoidance of somaclonal variation is of the feasibility for species for which tissue culture application hasn't been developed. VACUUM INFILTRATION was primarily applied in ARABIDOPSIS thaliana L. It has been used in several other species like radish. This investigation explains gene transfer into rapeseed using VACUUM INFILTRATION, for the first time in Iran. An ARABIDOPSIS inbred line was used as a check, and the frequencies of transgenic plants were compared. Two Agrobacterium strains, EHA101 and C58(pGV3101) containing pVW295 plasmid having NPTII and GDS GENEs were used in the investigation. The flowering rapeseed and ARABIDOPSIS plants were inverted into the agrobacterium culture in a vacuum chamber applying -350 to -400 mmHg of pressure for 30-40 seconds. Seeds from infiltrated rapeseed and ARABIDOPSIS plants were selected in media containing 50 and 30 mgL-1 Kanamycin respectively. To confirm the GUS gene transfer into the selected progenies histochemical evaluation was conducted. The histochemical assay confirmed the GDS GENE transfer. Evaluation of the infiltrated plant seeds revealed that the frequency of transgenic arabidosis was more than that in rapeseed. Agrobacterium strain EHA101 was more efficient than C58 (pGV3101) in gene transfer into either of arabidosisor rapeseed.

Cites

No record.

References

No record.

Cite

APA: Copy

GHORBANI MESHKIN, M., JALALI JAVARAN, MOKHTAR, & SEPAHVAND, N.A.. (2006). A STUDY OF GENE TRANSFORMATION BY VACUUM INFILTRATION INTO RAPESEED AND ARABIDOPSIS PLANTS. IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE), 37-1(3 (AGRONOMY & CROP BIOTECHNOLOGY)), 539-544. SID. https://sid.ir/paper/8639/en

Vancouver: Copy

GHORBANI MESHKIN M., JALALI JAVARAN MOKHTAR, SEPAHVAND N.A.. A STUDY OF GENE TRANSFORMATION BY VACUUM INFILTRATION INTO RAPESEED AND ARABIDOPSIS PLANTS. IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE)[Internet]. 2006;37-1(3 (AGRONOMY & CROP BIOTECHNOLOGY)):539-544. Available from: https://sid.ir/paper/8639/en

IEEE: Copy

M. GHORBANI MESHKIN, MOKHTAR JALALI JAVARAN, and N.A. SEPAHVAND, “A STUDY OF GENE TRANSFORMATION BY VACUUM INFILTRATION INTO RAPESEED AND ARABIDOPSIS PLANTS,” IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE), vol. 37-1, no. 3 (AGRONOMY & CROP BIOTECHNOLOGY), pp. 539–544, 2006, [Online]. Available: https://sid.ir/paper/8639/en

Related Journal Papers