STUDY OF GENE TRANSFORMABILITY ON COMMERCIAL OILSEED RAPE VARIETIES IN IRAN

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MASHAYEKHI MOHSEN; SHAKIB ALI MOHAMMAD; JALALI JAVARAN MOKHTAR; SOHEILIVAND SAEED; MOUSAVI AMIR

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Journal: Applied Agricultural Research Year:2012 | Volume:25 | Issue:1 (94) Page(s): 32-40

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Title

STUDY OF GENE TRANSFORMABILITY ON COMMERCIAL OILSEED RAPE VARIETIES IN IRAN

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Keywords

BRASSICA NAPUSQ2

GENE TRANSFORMATIONQ2

AGROBACTERIUMQ2

HYPOCOTYLQ2

COTYLEDONQ2

GUS ASSAYQ2

Abstract

Canola (BRASSICA NAPUS L.) is one of oil crops being cultivated in many areas of Iran. Its molecular breeding and production of varieties with new characteristics using genetic engineering needs the establishment of efficient transformation methods in commercial varieties. In this research transformation potential of 8 commercial cultivars; Licord, SLM046, RGS003, Zarfam, Okapi, Sarigol, Modena and Opera adapted to different regions of Iran was studied using COTYLEDON and HYPOCOTYL explants. AGROBACTERIUM tumifaciens, strain AGL0 containing the plasmid pCAMBIA3301 was used for transformation. COTYLEDON and HYPOCOTYL explants after inoculation with AGROBACTERIUM were co-cultivated on MS medium containing 1 mg/l 2, 4-D and 4.5 mg/l BAP, respectively. COTYLEDONary explants after co-cultivation were transferred on selection MS medium, containing 4.5 mg/l BAP and 3 mg/l phosphinothricin. HYPOCOTYL explants were transferred on selection MS medium containing 4 mg/l BAP, 2 mg/l Zeatin and 5 mg/l phosphinotricin. The regenerated plants were analyzed by PCR and histochemical GUS ASSAY for GENE TRANSFORMATION. The results showed that most of genotyes had GENE TRANSFORMATION potential regenerated from HYPOCOTYL segments, while this potential was limited to some cultivars using COTYLEDON explants. Among transgenic plants, using HYPOCOTYLs, Licord cultivar had the most transformation rate (15.26%) and Sarigol the least (0.2%). Also among regenerated plan's from COTYLEDON, SLM046 cultivar had the most transformation frequency (4.7%) and Modena, Opera and Zarfam cultivars did not respond to transformation.

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APA: Copy

MASHAYEKHI, MOHSEN, SHAKIB, ALI MOHAMMAD, JALALI JAVARAN, MOKHTAR, SOHEILIVAND, SAEED, & MOUSAVI, AMIR. (2012). STUDY OF GENE TRANSFORMABILITY ON COMMERCIAL OILSEED RAPE VARIETIES IN IRAN. APPLIED FIELD CROPS RESEARCH (PAJOUHESH & SAZANDEGI), 25(1 (94)), 32-40. SID. https://sid.ir/paper/214920/en

Vancouver: Copy

MASHAYEKHI MOHSEN, SHAKIB ALI MOHAMMAD, JALALI JAVARAN MOKHTAR, SOHEILIVAND SAEED, MOUSAVI AMIR. STUDY OF GENE TRANSFORMABILITY ON COMMERCIAL OILSEED RAPE VARIETIES IN IRAN. APPLIED FIELD CROPS RESEARCH (PAJOUHESH & SAZANDEGI)[Internet]. 2012;25(1 (94)):32-40. Available from: https://sid.ir/paper/214920/en

IEEE: Copy

MOHSEN MASHAYEKHI, ALI MOHAMMAD SHAKIB, MOKHTAR JALALI JAVARAN, SAEED SOHEILIVAND, and AMIR MOUSAVI, “STUDY OF GENE TRANSFORMABILITY ON COMMERCIAL OILSEED RAPE VARIETIES IN IRAN,” APPLIED FIELD CROPS RESEARCH (PAJOUHESH & SAZANDEGI), vol. 25, no. 1 (94), pp. 32–40, 2012, [Online]. Available: https://sid.ir/paper/214920/en

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