AN EVALUATION OF FIVE EUROPEAN PEARS (PYRUS COMMUNIS L.) INCOMPATIBILITY THROUGH MOLECULAR MARKER

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KOUSHESH SABA M.; ARZANI K.; JALALI JAVARAN MOKHTAR

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Journal: IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE) Year:2007 | Volume:38 | Issue:3 Page(s): 467-473

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Title

AN EVALUATION OF FIVE EUROPEAN PEARS (PYRUS COMMUNIS L.) INCOMPATIBILITY THROUGH MOLECULAR MARKER

Author(s)

KOUSHESH SABA M. | ARZANI K. | JALALI JAVARAN MOKHTAR | Issue Writer Certificate

Keywords

PEARQ3

PYNLS COMMUNIS L.Q2

SELF INCOMPATIBILITY (SI)Q2

MOLECULAR MARKERQ3

INCOMPATIBILITY ALLELESQ3

Abstract

Fruit set in PEAR is affected by several genetic and environmental factors one of which is incompatibility. Self-incompatibility (SI) in the Rosaceae is controlled by a homomorphic, gametophytic, monofactorial, multiallelic incompatibility system, where pollen rejection occurs in the style. The incompatibility locus controls self-incompatibility as well as intra-specific cross incompatibility.In the present study PCR along with specific primer was applied to investigate SI genes. This method is easy and fast and not affected by pollination factors. DNA from five European PEARs (Shah-Miveh, Spadona, Sardrood, Mohammad-Aliand Bartlet) was extracted using CTAB method. Specific primers amplified SI gene. The results showed that primers could amplify SI gene. S-locus was existent in the mentioned cultivars. The first pair of primers showed that there was one pair of SI, S3-S7, alleles in Shah-Mivehand in Mohammad-Ali. Sardrood and Bartlet had similar band patterns and had had S2 allele. In Spadona, Sardrood and Bartlet a 1400 base pair band was amplified which may be a new allele. The other primers resulted the same. Therefore, in order to obtain suitable yield one should pay attention to this fact and employ cultivars with different alleles in establishment of new PEAR orchards as well as in breeding programs.

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APA: Copy

KOUSHESH SABA, M., ARZANI, K., & JALALI JAVARAN, MOKHTAR. (2007). AN EVALUATION OF FIVE EUROPEAN PEARS (PYRUS COMMUNIS L.) INCOMPATIBILITY THROUGH MOLECULAR MARKER. IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE), 38(3), 467-473. SID. https://sid.ir/paper/8663/en

Vancouver: Copy

KOUSHESH SABA M., ARZANI K., JALALI JAVARAN MOKHTAR. AN EVALUATION OF FIVE EUROPEAN PEARS (PYRUS COMMUNIS L.) INCOMPATIBILITY THROUGH MOLECULAR MARKER. IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE)[Internet]. 2007;38(3):467-473. Available from: https://sid.ir/paper/8663/en

IEEE: Copy

M. KOUSHESH SABA, K. ARZANI, and MOKHTAR JALALI JAVARAN, “AN EVALUATION OF FIVE EUROPEAN PEARS (PYRUS COMMUNIS L.) INCOMPATIBILITY THROUGH MOLECULAR MARKER,” IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE), vol. 38, no. 3, pp. 467–473, 2007, [Online]. Available: https://sid.ir/paper/8663/en

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