IDENTIFICATION AND DISTRIBUTION OF POTATO SPINDLE TUBER VIROID (PSTVD) IN RAZAVI AND NORTHERN KHORASAN PROVINCES

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YAZAR LOU A.; JAFARPOUR BEHROUZ; FALAHATI RASTGAR M.

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Journal: Journal of Agricultural Sciences and Natural Resources Year:2008 | Volume:15 | Issue:1 Page(s): 97-104

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Title

IDENTIFICATION AND DISTRIBUTION OF POTATO SPINDLE TUBER VIROID (PSTVD) IN RAZAVI AND NORTHERN KHORASAN PROVINCES

Author(s)

YAZAR LOU A. | JAFARPOUR BEHROUZ | FALAHATI RASTGAR M. | Issue Writer Certificate

Keywords

POTATOQ2

PSTVDQ3

DENATURING POLYACRYLAMIDE-GEL ELECTROPHORESISQ3

RETURN ELECTROPHORESISQ3

Abstract

POTATO spindle tuber disease is one of the important diseases of POTATO. This viroid is an economically important pathogen especially in POTATO seed exporting countries, where they must produce certified and free PSTVD seeds. For detection of POTATO spindle tuber viroid in summer and fall of 2004, a survey in farms of Razavi and Northern Khorasan provinces was carried out and 250 tubers were collected that showing spindle shape and malformation symptoms. Samples were stored in cold room at 4oC. The tubers were planted under greenhouse condition after passing their dormancy period and starting germination. The leaves of these POTATOes were used for RNA extraction by PEG6000 precipitation method. After extraction, these RNA were loaded on the Denaturing Polyacrylamide Gel Electrophoresis at 60oC, and then stained by silver-staining manner. Specific viroid band in comparison with positive control was seen. In another procedure, viroid band has been revealed after loading RNA on Return Polyacrylamide Gel Electrophoresis at two temperatures, 15oC and 40oC, following silver staining procedure. The extracted RNA viroid was inoculated on healthy POTATOes (Agria cultivar) and tomatoes (Mobile cultivar) in greenhouse. Infection of inoculated plants has been confirmed by Return Polyacrylamide Gel Electrophoresis test as well. The symptoms mentioned above appeared on these plants after inoculation. This is the first report of PSTVD in Razavi and Northern Khorasan Provinces.

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APA: Copy

YAZAR LOU, A., JAFARPOUR, BEHROUZ, & FALAHATI RASTGAR, M.. (2008). IDENTIFICATION AND DISTRIBUTION OF POTATO SPINDLE TUBER VIROID (PSTVD) IN RAZAVI AND NORTHERN KHORASAN PROVINCES. JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, 15(1), 97-104. SID. https://sid.ir/paper/9579/en

Vancouver: Copy

YAZAR LOU A., JAFARPOUR BEHROUZ, FALAHATI RASTGAR M.. IDENTIFICATION AND DISTRIBUTION OF POTATO SPINDLE TUBER VIROID (PSTVD) IN RAZAVI AND NORTHERN KHORASAN PROVINCES. JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES[Internet]. 2008;15(1):97-104. Available from: https://sid.ir/paper/9579/en

IEEE: Copy

A. YAZAR LOU, BEHROUZ JAFARPOUR, and M. FALAHATI RASTGAR, “IDENTIFICATION AND DISTRIBUTION OF POTATO SPINDLE TUBER VIROID (PSTVD) IN RAZAVI AND NORTHERN KHORASAN PROVINCES,” JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES, vol. 15, no. 1, pp. 97–104, 2008, [Online]. Available: https://sid.ir/paper/9579/en

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