Study of total protein content, soluble sugar, proline content and P5CS gene expression in leaves of three wheat cultivars under drought stress

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Ghaderi Akram; JAHANBAKHSH GODEHKAHRIZ SODABEH; Raeisi sadati Seyede Yalda

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2021 | Volume:12 | Issue:4 Page(s): 125-144

Download Full-Text

Persian Verion

View:

493

Download:

Cites:

Information Journal Paper

Title

Study of total protein content, soluble sugar, proline content and P5CS gene expression in leaves of three wheat cultivars under drought stress

Author(s)

Ghaderi Akram | JAHANBAKHSH GODEHKAHRIZ SODABEH | Raeisi sadati Seyede Yalda | Issue Writer Certificate

Keywords

Drought Stress

Proline

P5CS Gene

Total Protein

Wheat

Abstract

Objective The present study was designed to identify the physiological and molecular responses of Drought Stress induced metabolism in plants. Since the studied plants include resistant and susceptible Wheat crop, it is important to know the drought tolerance mechanisms in these cultivars in order to improve the resistance and tolerance of Drought Stress to the climate in Iran. Materials and Methods For this purpose, a factorial experiment based on completely randomized design with three replications was conducted in Mohaghegh Ardebil University in 2018-2019. The main factors included Drought Stress (35, 60 and 85% of field capacity (control)) and sub factor included three Wheat cultivars (Pishgam, Pishtaz and Baharan). Drought Stress in the three-leaf stage was applied for 10 days and then the seedlings were sampled to check the Total Protein content of the solution, the sugar content, Proline content and the expression of Proline-5-D gene expression-5-carboxyxyl synthase (P5CS) of the leaf tissue sample. Results The results showed that with increasing severity of Drought Stress, the amount of Proline, soluble sugar and Total Protein increased. The highest and lowest levels of Total Protein, respectively, belonged to the leading cultivar under conditions of strict control and stress. The highest amount of soluble sugar (75. 76 and 91. 66 mg / g, respectively, wet weight of leaves) in spring Wheat and severe Drought Stress and the lowest (57. 59 and 48. 7 mg / g, respectively). Examination of P5SC gene expression showed that the number of transcripts of this gene increases under stress and this shows that this gene plays an important role in responding to stress in plants. Baharan showed higher drought resistance than other cultivars by increasing P5CS Gene expression and significant Proline production and accumulation under Drought Stress. Conclusions In general, it is inferred that drought-induced expression key gene involved in the biosynthesis of Proline (P5CS) cause increased levels of Proline leaves and also with the accumulation of soluble sugar probably causes more stress tolerance in cultivars.

Cites

No record.

References

No record.

Cite

APA: Copy

Ghaderi, Akram, JAHANBAKHSH GODEHKAHRIZ, SODABEH, & Raeisi sadati, Seyede Yalda. (2021). Study of total protein content, soluble sugar, proline content and P5CS gene expression in leaves of three wheat cultivars under drought stress. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 12(4 ), 125-144. SID. https://sid.ir/paper/958696/en

Vancouver: Copy

Ghaderi Akram, JAHANBAKHSH GODEHKAHRIZ SODABEH, Raeisi sadati Seyede Yalda. Study of total protein content, soluble sugar, proline content and P5CS gene expression in leaves of three wheat cultivars under drought stress. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2021;12(4 ):125-144. Available from: https://sid.ir/paper/958696/en

IEEE: Copy

Akram Ghaderi, SODABEH JAHANBAKHSH GODEHKAHRIZ, and Seyede Yalda Raeisi sadati, “Study of total protein content, soluble sugar, proline content and P5CS gene expression in leaves of three wheat cultivars under drought stress,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 12, no. 4 , pp. 125–144, 2021, [Online]. Available: https://sid.ir/paper/958696/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops