IgY production in egg yolk against Salmonella typhimurium and salmonella enteritidis

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Eshaghi Shima; NASSIRI MOHAMMAD REZA; TAHMOORESPOUR MOJTABA; JAVADMANESH ALI

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Journal: JOURNAL OF AGRICULTURAL BIOTECHNOLOGY Year:2020 | Volume:12 | Issue:3 Page(s): 177-190

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Information Journal Paper

Title

IgY production in egg yolk against Salmonella typhimurium and salmonella enteritidis

Author(s)

Eshaghi Shima | NASSIRI MOHAMMAD REZA | TAHMOORESPOUR MOJTABA | JAVADMANESH ALI | Issue Writer Certificate

Keywords

Laying hens

IgY

Salmonella

Egg yolk

Abstract

Objective Salmonella is a Gram-negative, anaerobic, flagellated bacterium of Salmonella type in Enterobacteriaceae family, which is known as a zoonosis infectious agent. In recent years, different types of antibiotics have been used to overcome the Salmonella infection; however, the increasing number of antibiotic-resistant bacteria, the provision of alternative methods seems be necessary. Specific immunoglobulin Y (IgY) originated from Egg yolk causes passive immunity in the fetus, and extracting these antibodies from Egg yolk is cheaper and more feasible than other antibodies. Numerous studies have shown that the Egg yolk IgY has immunogenic function against a wide range of bacterial and viral infections in mammals. The purpose of this study was to produce specific immunoglobulin Y against Salmonella typhimurium and Salmonella enteritidis bacteria in Laying hens. Materials and Methods In the current study, six pullets were inoculated and boosted with one milliliter of killed Salmonella typhimurium and Salmonella enteritidis. Injections were done fortnightly until one week before egg production. After breast muscle injection of chickens, the eggs were collected, and the IgY was extracted from yolk by the Polson method with polyethylene glycol 6000. The produced IgY was approved by SDS-PAGE method. The specific indirect ELISA done by specificity test for purified IgY also approved the specific IgY against both Salmonella typhimurium and Salmonella enteritidis. Results The results of SDS-PAGE demonstrated the presence of two protein bands of 27 and 67 kDa representing light and heavy chains of IgY, respectively. The specific indirect ELISA method proved that the specific IgY against Salmonella typhimurium and Salmonella enteritidis was produced in the Egg yolk. Conclusions It might be possible to administer the Egg yolk containing specific IgY against both Salmonella typhimurium and Salmonella enteritidis, to induce passive immunity for preventing some pathogenic diseases in calves such as diarrhea.

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APA: Copy

Eshaghi, Shima, NASSIRI, MOHAMMAD REZA, TAHMOORESPOUR, MOJTABA, & JAVADMANESH, ALI. (2020). IgY production in egg yolk against Salmonella typhimurium and salmonella enteritidis. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, 12(3 ), 177-190. SID. https://sid.ir/paper/961739/en

Vancouver: Copy

Eshaghi Shima, NASSIRI MOHAMMAD REZA, TAHMOORESPOUR MOJTABA, JAVADMANESH ALI. IgY production in egg yolk against Salmonella typhimurium and salmonella enteritidis. JOURNAL OF AGRICULTURAL BIOTECHNOLOGY[Internet]. 2020;12(3 ):177-190. Available from: https://sid.ir/paper/961739/en

IEEE: Copy

Shima Eshaghi, MOHAMMAD REZA NASSIRI, MOJTABA TAHMOORESPOUR, and ALI JAVADMANESH, “IgY production in egg yolk against Salmonella typhimurium and salmonella enteritidis,” JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 12, no. 3 , pp. 177–190, 2020, [Online]. Available: https://sid.ir/paper/961739/en

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