PRODUCTION OF MONOCLONAL ANTIBODY AGAINST THE NUCLEOCAPSID (N) PROTEIN OF INFECTIOUS BRONCHITIS VIRUS (IBV)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

RAMESH B.; SEYFI ABAD SHAPOURI M.R.; GHORBANPOOR M.; MEHRAGHA M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL Year:2016 | Volume:12 | Issue:3 (52) Page(s): 42-50

Download Full-Text

Persian Verion

View:

803

Download:

Cites:

Information Journal Paper

Title

PRODUCTION OF MONOCLONAL ANTIBODY AGAINST THE NUCLEOCAPSID (N) PROTEIN OF INFECTIOUS BRONCHITIS VIRUS (IBV)

Author(s)

RAMESH B. | SEYFI ABAD SHAPOURI M.R. | GHORBANPOOR M. | MEHRAGHA M. | Issue Writer Certificate

Keywords

INFECTIOUS BRONCHITIS VIRUSQ2

NUCLEOCAPSID PROTEINQ1

PROTEIN EXPRESSIONQ1

MONOCLONAL ANTIBODYQ1

Abstract

INFECTIOUS BRONCHITIS VIRUS (IBV) is an important viral pathogen of chickens with several serotypes. Efficient control of the infections caused by IBV relay on the vaccination and rapid diagnosis of infections. The nucleocapsid (N) protein of the virus is a conserved structural protein, considered as a candidate antigen for developing diagnostic kits and production of monoclonal antibodies. In the present study the N protein of INFECTIOUS BRONCHITIS VIRUS, H120 vaccine strain was expressed in Escherichia coli in order to be used as antigen for the production of monoclonal antibodies. Two monoclonal antibodies (1F9 and 6C3) reactive against the recombinant N protein were produced and tested for their reaction with the native viral N protein by Western blotting and immunodot. Both antibodies recognized the N protein in Western blotting but only 1F9 antibody was positive in immunodot.1F9 antibody also reacted with 4.91 and QX stains of IBV. It appears this MONOCLONAL ANTIBODY possess the diagnostic application.

Cites

No record.

References

No record.

Cite

APA: Copy

RAMESH, B., SEYFI ABAD SHAPOURI, M.R., GHORBANPOOR, M., & MEHRAGHA, M.. (2016). PRODUCTION OF MONOCLONAL ANTIBODY AGAINST THE NUCLEOCAPSID (N) PROTEIN OF INFECTIOUS BRONCHITIS VIRUS (IBV). SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 12(3 (52)), 42-50. SID. https://sid.ir/paper/97212/en

Vancouver: Copy

RAMESH B., SEYFI ABAD SHAPOURI M.R., GHORBANPOOR M., MEHRAGHA M.. PRODUCTION OF MONOCLONAL ANTIBODY AGAINST THE NUCLEOCAPSID (N) PROTEIN OF INFECTIOUS BRONCHITIS VIRUS (IBV). SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2016;12(3 (52)):42-50. Available from: https://sid.ir/paper/97212/en

IEEE: Copy

B. RAMESH, M.R. SEYFI ABAD SHAPOURI, M. GHORBANPOOR, and M. MEHRAGHA, “PRODUCTION OF MONOCLONAL ANTIBODY AGAINST THE NUCLEOCAPSID (N) PROTEIN OF INFECTIOUS BRONCHITIS VIRUS (IBV),” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 12, no. 3 (52), pp. 42–50, 2016, [Online]. Available: https://sid.ir/paper/97212/en

Related Journal Papers