EARLY DIAGNOSIS OF SHEEP FASCIOLOSIS WITH ENZYME LINKED IMMUNOSORBANT ASSAY (ELISA)

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PEYKARI H.; ABSHAR N.; MOTAMEDI G.R.; DARAKHSHANFAR M.; KARIMI G.R.

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Journal: SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL Year:2006 | Volume:2 | Issue:2 (13) Page(s): 64-73

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Title

EARLY DIAGNOSIS OF SHEEP FASCIOLOSIS WITH ENZYME LINKED IMMUNOSORBANT ASSAY (ELISA)

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Keywords

FASCIOLA GIGANTICAQ3

EXPERIMENTAL INFECTIONQ4

ELISAQ2

DIAGNOSISQ2

Abstract

Early DIAGNOSIS of fasciolosis in livestock is important for timely prevention of the disease and economic losses. In this study, ELISA method was employed for early DIAGNOSIS of infection. For this purpose, 50 sheep of less than one year of age were infected experimentally with 100 metacercaria of FASCIOLA GIGANTICA, while Lymnaea gedrosiana was used as an intermediate host in this experiment. Blood was drawn from the sheep at 1st, 2nd and 3rd weeks prior to infection; also at months 1 to 6 after the infection. As a control, the serum of 50 sheep prior to their infection with FASCIOLA GIGANTICA, and 15 sheep infected with hydatid cyst and another 15 infected with Ornitobilharzia turkestanicum were used. For ELISA, 5 mg of excretory-secretary antigens and 1: 10000 Anti-sheep IgG peroxidase conjugate were used. The serum was diluted to 1:200 and cut-off OD of 0.743 was selected for these tests. The results indicated that in third week of infection the disease was diagnosed and 94% of the cases were appeared positive, while in the twelfth week, the antibody titer was at its maximum. The sensitivity and specificity of the test were 96% and 83.7%, respectively.

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APA: Copy

PEYKARI, H., ABSHAR, N., MOTAMEDI, G.R., DARAKHSHANFAR, M., & KARIMI, G.R.. (2006). EARLY DIAGNOSIS OF SHEEP FASCIOLOSIS WITH ENZYME LINKED IMMUNOSORBANT ASSAY (ELISA). SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 2(2 (13)), 64-73. SID. https://sid.ir/paper/97316/en

Vancouver: Copy

PEYKARI H., ABSHAR N., MOTAMEDI G.R., DARAKHSHANFAR M., KARIMI G.R.. EARLY DIAGNOSIS OF SHEEP FASCIOLOSIS WITH ENZYME LINKED IMMUNOSORBANT ASSAY (ELISA). SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2006;2(2 (13)):64-73. Available from: https://sid.ir/paper/97316/en

IEEE: Copy

H. PEYKARI, N. ABSHAR, G.R. MOTAMEDI, M. DARAKHSHANFAR, and G.R. KARIMI, “EARLY DIAGNOSIS OF SHEEP FASCIOLOSIS WITH ENZYME LINKED IMMUNOSORBANT ASSAY (ELISA),” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 2, no. 2 (13), pp. 64–73, 2006, [Online]. Available: https://sid.ir/paper/97316/en

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