THE STUDY OF TISSUE STRUCTURE IN THE SKIN OF ARGYROSOMUS HOLOLEPIDOTUSBY LIGHT AND ELECTRON MICROSCOPICS

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MOROVVATI H.; ESFANDIARI K.; SHEYBANI M.; TOTIAN Z.

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Paper Information

Journal: SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL Year:2016 | Volume:11 | Issue:4 Page(s): 62-77

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Title

THE STUDY OF TISSUE STRUCTURE IN THE SKIN OF ARGYROSOMUS HOLOLEPIDOTUSBY LIGHT AND ELECTRON MICROSCOPICS

Author(s)

MOROVVATI H. | ESFANDIARI K. | SHEYBANI M. | TOTIAN Z. | Issue Writer Certificate

Keywords

HISTOLOGYQ2

HISTOCHEMISTRYQ2

SKINQ2

ARGYROSOMUS HOLOLEPIDOTUSQ2

Abstract

One of the most valuable fish in Persian Gulf, Oman Sea, and territorial (coastal) waters of Khozestan, a province in the southwest of Iran, is ARGYROSOMUS HOLOLEPIDOTUS. In fish, SKIN is the first line of defense against the external environment and furthermore makes possible natural physiological functions inside the body. In this study, six ARGYROSOMUS HOLOLEPIDOTUS were used for microscopic analyses. The samples were prepared from 0.5μm cuts from the fish dorsum and later they were dyed in toluidine blue, H & E, PAS, AB (pH=2.5) and AB (pH=2.5) -PAS. For transmission electron microscopic analyses, the samples were dehydrated and embedded in resin after primary and post-fixation. Later, cuts with the thickness between 50nm to 80nm were prepared and dyed in uranyl acetate. The result of the light microscopic analysis showed that the fish’s epidermis is composed from goblet, epithelial, and superficial- squamous cells. Their dermis contains spongiosum and compactum layers. The subcutis does not exist in some parts and it is thin in some parts. The results of the electron microscopic analyses showed that goblet cells include mucosal drops. The superficial squamous cells have micro ridges that keep in touch thorough tight junctions and the cytoplasm of epithelial epidermis cells has filaments that keep in touch via desmosomes. In addition, the result of histochemical analysis showed that goblet cells, superficial squamous cells and epithelial cells reacted positively to PAS and AB dyes with pH 2.5.

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APA: Copy

MOROVVATI, H., ESFANDIARI, K., SHEYBANI, M., & TOTIAN, Z.. (2016). THE STUDY OF TISSUE STRUCTURE IN THE SKIN OF ARGYROSOMUS HOLOLEPIDOTUSBY LIGHT AND ELECTRON MICROSCOPICS. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 11(4), 62-77. SID. https://sid.ir/paper/97347/en

Vancouver: Copy

MOROVVATI H., ESFANDIARI K., SHEYBANI M., TOTIAN Z.. THE STUDY OF TISSUE STRUCTURE IN THE SKIN OF ARGYROSOMUS HOLOLEPIDOTUSBY LIGHT AND ELECTRON MICROSCOPICS. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2016;11(4):62-77. Available from: https://sid.ir/paper/97347/en

IEEE: Copy

H. MOROVVATI, K. ESFANDIARI, M. SHEYBANI, and Z. TOTIAN, “THE STUDY OF TISSUE STRUCTURE IN THE SKIN OF ARGYROSOMUS HOLOLEPIDOTUSBY LIGHT AND ELECTRON MICROSCOPICS,” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 11, no. 4, pp. 62–77, 2016, [Online]. Available: https://sid.ir/paper/97347/en

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