DETERMINATION OF NECROTOXIGENIC ESCHERICHIA COLI ISOLATES IN THE CASES OF GASTROINTESTINAL INFECTIONS OF CALVES IN KERMAN REGION

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GHANBARPOUR REZA; KARNAMA F.

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Journal: SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL Year:2009 | Volume:4 | Issue:5 (22) Page(s): 49-56

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Title

DETERMINATION OF NECROTOXIGENIC ESCHERICHIA COLI ISOLATES IN THE CASES OF GASTROINTESTINAL INFECTIONS OF CALVES IN KERMAN REGION

Author(s)

GHANBARPOUR REZA | KARNAMA F. | Issue Writer Certificate

Keywords

ESCHERICHIA COLIQ2

NECROTOXIGENICQ3

CYTOTOXIC NECROTIZING FACTORQ3

CYTOLETHAL DISTENDING TOXINQ3

CALFQ3

Abstract

The purpose of this study was to determine the NECROTOXIGENIC E. coli isolates and their O serogroup from calves with gastrointestinal tract infections. Rectal swabs were obtained from 163 calves and streaked on Mac Conkey agar for isolation of E. coli. Suspected colonies were cultured in biochemical media to confirm the isolates. One confirmed colony from each sample was cultured in Luria Bertani broth (LB) and stored with 30% glycerol at -20°c. Multiplex PCR were undertaken to determine the positive isolates for cnf and cdt families genes encoding CYTOTOXIC NECROTIZING FACTORs and CYTOLETHAL DISTENDING TOXINs respectively. The positive isolates in the first step were used in several simplexes PCR to detect the variants of cnf and cdt genes. The NECROTOXIGENIC isolates were also sero-grouped. According to the results, 138 E. coli were isolated from 163 samples. In the PCRs of the first step, 12 (8.69%) isolates had the 1150 and 466 base pair bands which were positive for cnf and cdt family's genes. All of the 12 positive isolates showed the presence of cnf2 and cdtIII genes and considered as NECROTOXIGENIC E. coli 2 (NTEC2). None of the isolates was positive for cnf1 gene which was found in NECROTOXIGENIC E. coli 1 (NTEC 1) isolates. Serogroup determination of NTEC2 isolates showed that three isolates belonged to 0 IS, two isolates to 078 and one isolates to 08; the remaining of isolates (6) were non-typeable by standard antisera. In most studies in other parts of world, NTEC2 strains were isolated from calves that belonged to different O serogroups.

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APA: Copy

GHANBARPOUR, REZA, & KARNAMA, F.. (2009). DETERMINATION OF NECROTOXIGENIC ESCHERICHIA COLI ISOLATES IN THE CASES OF GASTROINTESTINAL INFECTIONS OF CALVES IN KERMAN REGION. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 4(5 (22)), 49-56. SID. https://sid.ir/paper/97447/en

Vancouver: Copy

GHANBARPOUR REZA, KARNAMA F.. DETERMINATION OF NECROTOXIGENIC ESCHERICHIA COLI ISOLATES IN THE CASES OF GASTROINTESTINAL INFECTIONS OF CALVES IN KERMAN REGION. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2009;4(5 (22)):49-56. Available from: https://sid.ir/paper/97447/en

IEEE: Copy

REZA GHANBARPOUR, and F. KARNAMA, “DETERMINATION OF NECROTOXIGENIC ESCHERICHIA COLI ISOLATES IN THE CASES OF GASTROINTESTINAL INFECTIONS OF CALVES IN KERMAN REGION,” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 4, no. 5 (22), pp. 49–56, 2009, [Online]. Available: https://sid.ir/paper/97447/en

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