THE SURVEY OF COW'S PREGNANCY DIAGNOSIS ACCURANCE WITH MEASUREMENT OF MILK PROGESTERONE BY ELISA

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MOSAFERI S.; NICRAFTAR SAHANDI Y.; GHAFARI LALEH V.

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Journal: SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL Year:2007 | Volume:3 | Issue:1 (14) Page(s): 71-77

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Title

THE SURVEY OF COW'S PREGNANCY DIAGNOSIS ACCURANCE WITH MEASUREMENT OF MILK PROGESTERONE BY ELISA

Author(s)

MOSAFERI S. | NICRAFTAR SAHANDI Y. | GHAFARI LALEH V. | Issue Writer Certificate

Keywords

COWQ3

ELISAQ2

PROGESTERONEQ3

PREGNANCY DETECTIONQ3

MILKQ2

Abstract

An accurate, early diagnosis of pregnancy in the COW has become essential for a successful, and profite breeding program. Between different methods of pregnancy diagnosis assessment of serum or MILK PROGESTERONE is one of the best method for early diagnosis of pregnancy in dairy herd. PROGESTERONE can be measured by ELISA and RIA methods. ELISA was preferred to RIA for being more accurate, cheaper, safer, less time consuming and also needs less laboratory equipments. In this study, MILK samples (10-20ml) were collected from evening MILKing in clean test tubes and kept out from light and heat and submitted to the lab as soon as possible. Then its fat homogenized by shaker and its PROGESTERONE content determined by ELISA. If the PROGESTERONE value was more than 2ng/ml, the pregnancy is confirmed and less than 2ng/ml was reported as negative. In this four month survey, the samples were collected from 272 dairy COWs of industrial farms in the vicinity of Tabriz, which were inseminated 19, 20, 21, 22, 23 and 24 days later. Then, the PROGESTERONE contents of the samples were determined in laboratory.From the 272 samples taken, 76 cases were determined non pregnant by ELISA, and this 76 cases were also checked for pregnancy by ultrasonography and reported non pregnant in 35 days after insemination. Therefore, the accuracy of this method in negative cases was 100%. From 196 cases considered positive through PROGESTERONE measuring method, 136 cases were pregnant and 60 cases were detected non pregnant by ultrasonography in 35 days so that the accuracy of this method for diagnosis of pregnant animal was 69.4 %. In general the accuracy rate of diagnosis pregnancy was 84.7 % and the rate of accuracy of this method was in nineteen days 78.5 %, twenty days 78.5 %, twenty one days 81.2 %, twenty two days 96.8 %, twenty three days 87.5%, and twenty four days 81.8 %. Although the accuracy of this method in nineteen days after insemination was 78.5%, on that day there was sufficient opportunity for detecting of estrus and rebreeding of animal. However, the best time for detecting pregnancy in this method was 22 days after insemination with accuracy rate of 96.5%.

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APA: Copy

MOSAFERI, S., NICRAFTAR SAHANDI, Y., & GHAFARI LALEH, V.. (2007). THE SURVEY OF COW'S PREGNANCY DIAGNOSIS ACCURANCE WITH MEASUREMENT OF MILK PROGESTERONE BY ELISA. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 3(1 (14)), 71-77. SID. https://sid.ir/paper/97475/en

Vancouver: Copy

MOSAFERI S., NICRAFTAR SAHANDI Y., GHAFARI LALEH V.. THE SURVEY OF COW'S PREGNANCY DIAGNOSIS ACCURANCE WITH MEASUREMENT OF MILK PROGESTERONE BY ELISA. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2007;3(1 (14)):71-77. Available from: https://sid.ir/paper/97475/en

IEEE: Copy

S. MOSAFERI, Y. NICRAFTAR SAHANDI, and V. GHAFARI LALEH, “THE SURVEY OF COW'S PREGNANCY DIAGNOSIS ACCURANCE WITH MEASUREMENT OF MILK PROGESTERONE BY ELISA,” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 3, no. 1 (14), pp. 71–77, 2007, [Online]. Available: https://sid.ir/paper/97475/en

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