Nutritional balance of crops is one of the most important factors which affect the quantitative and qualitative yield of crops. The method of deviation from optimum percentage (DOP) is one of the suitable methods in interpret the results of the nutrients analysis in crops, the nutrients requirements and the nutritional balance status in crops. The objective of this study was to evaluate the nutritional status of sugarcane by the DOP method in 25 plant fields, 72 first ratoon fields and 44 second ratoon fields in Hakim Farabi Agro Industry Company, Khuzestan province. Leaf samples were collected from studied fields and concentration of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), manganese (Mn), zinc (Zn) and copper (Cu) were measured. In order to interpret the nutrient concentration data and assess the nutritional status of sugarcane, the fields were divided into two groups with high and low yield. Then nutrient norms, nutrient DOP indices and nutritional balance index (∑DOP) were calculated. The results indicated that in the low yield fields, K and P (as macronutrients) had more negative indices, as well as Fe and Zn (as micronutrients). Based on DOP indices, the order of crop nutrients requirement in low yield fields were as K>P>Mg>Ca>N, K>P>N>Mg>Ca and P>K>N>Mg>Ca in plant, first ratoon and second ratoon, respectively. In general, fertilizer inappropriate management resulted to the imbalance of nutritional in sugarcane that it affect sugarcane yield. Therefore, balanced and optimal fertilization with integrated application of chemical, organic and biological fertilizers should be considered in sugarcane fields.