In this study, various statistical methodologies, including Additive Main effects and Multiplicative Interaction (AMMI) and Best Linear Unbiased Prediction (BLUP), were employed to identify high-yielding rainfed barley genotypes that are suitable for the cold and rainy regions of Iran. The experimental design comprised 25 barley cultivars and lines, along with three check cultivars, arranged in a randomized complete block design with four replications over three crop years (2017-2020). The AMMI analysis revealed that certain genotypes, specifically G15 and G21, demonstrated stability and adaptability across diverse environments, consistently yielding higher than other genotypes. Following the estimation of best linear unbiased predictions and conducting a stability analysis via the AMMI method, it was found that the highest yields were recorded in genotypes G6, G7, G15, G21, and G22, whereas the lowest yields were associated with genotypes G12, G25, G26, G27, and G28. According to the BLUP indices, genotypes G6, G15, G21, G20, G22, G17, G7, G9, and G19 were identified as superior in terms of grain stability and yield relative to the other genotypes. In the stability assessment utilizing a third-type biplot (yield versus WAASB (Weighted Average of Absolute Scores of the Best) index), it was noted that genotypes G2, G9, G10, G14, G16, G17, G19, G20, and G22 exhibited both high yield and stability. Furthermore, genotypes G4, G62, G7, G9, G10, G15, G16, G17, G19, G20, G21, and G22, which demonstrated the highest WAASBY (Weighted Average of Absolute Scores of the Best Yield) values, were classified as stable and high-yielding. Ultimately, when the first principal components in the AMMI analysis or GGE Biplot account for a lower percentage of genotype-environment interaction, it is advisable to employ methodologies that incorporate all significant principal components to effectively identify superior genotypes.