Background: Hepatic lipase (HL) plays a crucial role in lipid metabolism, but there is debate about whether HL acts in a more pro-or more anti-atherogenic fashion. We aimed to examine the relationship between the-514 C/T polymorphism within the HL gene (LIPC) and the risk of angiographically determined premature coronary artery disease (CAD). Methods: Four hundred seventy-one patients with newly diagnosed angiographically documented (≥ 50% luminal stenosis of any coronary vessel) premature CAD were compared to 503 controls (subjects with no luminal stenosis in coronary arteries). A real-time polymerase chain reaction and high-resolution melting analysis was used to distinguish between the genotypes. Results: There was no significant difference in the distribution of-514 C/T genotypes between the 2 groups in the whole population or in the men, but the examined polymorphism was found to be associated with the presence of CAD in the women (p value = 0. 029). After the application of a multiple logistic regression model, the minor T allele of the LIPC gene was not found to be independently associated with the presence of CAD either in the total population (adjusted OR = 0. 97, 95% CI = 0. 75-1. 25; p value = 0. 807) or in the women (adjusted OR = 0. 91, 95% CI = 0. 59-1. 40; p value = 0. 650) and in the men (adjusted OR = 1. 15, 95% CI = 0. 81-1. 64; p value = 0. 437) separately. Conclusion: Our findings suggest that there is no relationship between the LIPC-514 C/T and the risk of premature CAD or its severity in patients undergoing coronary angiography.

Introduction: Genetic load may indirectly influence on cardiovascular risk. This work aimed to analyze the influence of polymorphisms (APOA5 C56G‑ Ser19Trp, Prothrombin‑ G20210A, F5 Arg506Gln, MTHFR‑ C677T, LIPC‑ C‑ 514T, LPA‑ I4300M, PPAR_ALPHA‑ L162V, APOA5‑ 1131T > C, APOE‑ APOE2/3/4, and APOE‑ APOE2, 3, 4) in plasma triglyceride (TG) levels of patients ingesting plant sterols. Materials and Methods: Double‑ blind, crossover, controlled clinical trial was performed in 45 individuals (25 women). About 2. 24 g sterols in milk and placebo milk were ingested daily during 3 weeks each, separated by a 2‑ week washout period. Blood draws and saliva genomic DNA was extracted. Results: APOA5‑ C56G‑ Ser19Trp, MTHFR‑ C677T, and PPAR_ALPHA‑ L162V greatly benefit from sterols intake. APOA5‑ C56G‑ Ser19Trp GG homozygous carriers lowered their TGs more than CG heterozygote carriers (P = 0. 003). TT homozygous carriers of gene LIPC C‑ 514T experienced an increase of TGs. Conclusions: Further studies are needed to establish which genotype combinations is the most protective against hypertriglyceridemia.

Background: The T allele of the hepatic lipase (HL) C-514T polymorphism was previously found to be associated with lower plasma HL activity. Here, we examined the association between this polymorphism and plasma HDL-cholesterol concentrations in patients with coronary arteries stenosis.Methods: We studied 342 subjects undergoing coronary angiography in two groups of non CAD (n=146) and CAD (n=196). -514C®T polymorphism was determined using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).Results: After adjustment for age, smoking and body mass index, HDL-cholesterol concentrations were significantly higher in men with the C/T& T/T genotype than those with the C/C genotype (mean 38.6 and 34.7 respectively P=0.01). The frequency of T allele in non CAD was 0.136 and 0.226 in female and male respectively and 0.170 and 0.223 for female and male in CAD subjects. There was no difference in T allele frequency in CAD and none CAD groups in male and female (P=0.466 and 0.722 respectively).Conclusion: -514C®T of LIPC gene have a positive effect on HDL-C concentration especially in male gender. However, no difference was determined in frequency of T allele between CAD and normal arteries subjects.